Comparing gene expression profiles of Kashin-Beck and Keshan diseases occurring within the same endemic areas of China  

作　　者：WANG Xi WANG Shuang HE ShuLan ZHANG Feng TAN WuHong LEI YanXia YU HanJie LI Zheng NING YuJie XIANG YouZhang GUO Xiong 

机构地区：[1]Faculty of Public Health, Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry of Education, Key Laboratory of Trace Elements and Endemic Diseases of Ministry of Health [2]National Engineering Research Center for Miniaturized Detection Systems, Northwest University [3]Shandong Institute for Prevention & Treatment of Endemic Disease

出　　处：《Science China(Life Sciences)》2013年第9期797-803,共7页中国科学（生命科学英文版）

基　　金：supported in part by the Key Scientific and Technological Innovation Special Projects of Shaanxi "13115" of China (2009ZDKG-79);the National Natural Science Foundation of China (30872192, 81273008)

摘　　要：In this study, differentially expressed genes in peripheral blood from patients with Kashin-Beck disease and Keshan disease were compared to further investigate the etiology and pathogenesis of both diseases, which occur in a common endemic area of China. Twenty Kashin-Beck disease patients and 12 healthy controls, and 16 Keshan disease patients and 16 healthy controls, were grouped into four pairs. Patients and controls were selected from common endemic areas for the two diseases. Total RNA was isolated from peripheral blood mononuclear cells from all patients and controls, and gene expression profiles analyzed by oligonucleotide microarrays. Sixteen genes differentially expressed in both Kashin-Beck disease and Keshan disease (versus controls) were identified, and comprised nine genes showing synchronous and seven asynchronous expression. The Comparative Toxicogenomics Database shows that expression and biological function of these genes can be affected by multiple environmental factors, including mycotoxin and selenium content, potential environmental risk factors for the two diseases. Thus, these shared differentially expressed genes may contribute to the distinct organ lesions, caused by common environmental risk factors of Kashin-Beck disease and Keshan disease.In this study, differentially expressed genes in peripheral blood from patients with Kashin-Beck disease and Keshan disease were compared to further investigate the etiology and pathogenesis of both diseases, which occur in a common endemic area of China. Twenty Kashin-Beck disease patients and 12 healthy controls, and 16 Keshan disease patients and 16 healthy controls, were grouped into four pairs. Patients and controls were selected from common endemic areas for the two diseases. Total RNA was isolated from peripheral blood mononuclear cells from all patients and controls, and gene expression profiles analyzed by oligonucleotide microarrays. Sixteen genes differentially expressed in both Kashin-Beck disease and Keshan disease （versus controls） were identified, and comprised nine genes showing synchronous and seven asynchronous expression. The Comparative Toxicogenomics Database shows that expression and biological function of these genes can be affected by multiple environmental factors, including mycotoxin and selenium content, potential environmental risk factors for the two diseases. Thus, these shared differentially expressed genes may contribute to the distinct organ lesions, caused by common environmental risk factors of Kashin-Beck disease and Keshan disease.

关 键 词：Kashin-Beck disease Keshan disease MICROARRAY peripheral blood mononuclear cells common environmental factors differential gene expression 

分 类 号：R684.1[医药卫生—骨科学] R542.3[医药卫生—外科学]