老龄贵州小型猪胃黏膜5-HT-IR及SS-IR细胞的初步观察  

Preliminary observation of SS-IR cell and 5-HT on gastric mucosa of Guizhou miniature aged pigs

作　　者：吴曙光[1] 刘霞[2] 王明镇[1] 詹新义[1] 周谷于[3] 钱宁[1] 

机构地区：[1]贵阳中医学院实验动物研究所,贵州贵阳550002 [2]贵阳中医学院组织学与胚胎学教研室,贵州贵阳550002 [3]贵阳中医学院基础医学院,贵州贵阳550002

出　　处：《西北农林科技大学学报（自然科学版）》2013年第8期38-41,48,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金："十五"国家科技攻关计划重点项目(2004BA717B-01-03);贵州省科技计划项目(黔科合中药字[P2012]LKZ7001号)

摘　　要：【目的】观察、比较分析老龄贵州小型猪胃内分泌细胞的形态、数量和分布。【方法】采集老龄和青年贵州小型猪的胃,取胃的不同部位(贲门、胃体、胃窦),用免疫组织化学染色法分别对老龄和青年贵州小型猪胃黏膜中的5-羟色胺(5-HT)、生长抑素(SS)细胞染色,在400倍视野中观察其形态及分布,并进行计数,比较老龄与青年贵州小型猪胃黏膜的差异。【结果】老龄和青年贵州小型猪胃黏膜中的5-HT-IR、SS-IR阳性细胞呈锥形、圆形、卵圆形等多种形态;分布不均匀,分别主要分布于胃腺的体部、底部或黏膜表面上皮细胞之间。老龄贵州小型猪胃窦5-HT-IR阳性细胞数较贲门极显著增加(P<0.01),胃体较贲门显著增加(P<0.05);胃窦SS-IR阳性细胞数较贲门极显著增加(P<0.01),而胃体与贲门差异不显著。老龄贵州小型猪5-HT-IR、SS-IR阳性细胞数较青年贵州小型猪明显减少,且差异达极显著水平(P<0.01)。【结论】老龄贵州小型猪胃黏膜5-HT-IR、SS-IR阳性细胞的形态、分布规律与青年贵州小型猪相似,但分泌5-HT、SS的细胞数量减少,提示老龄贵州小型猪的胃黏膜内分泌功能减退。This study aimed to observe and compare the morphology,number and distribution of gastric endocrine cells of aged and young Guizhou miniature pigs.【Method】Stomachs of aged and young Guizhou miniature pigs were collected and different parts（cardiac,gastric body and antral） of their stomachs were picked.5-HT-IR and SS-IR cells on gastric mucosa were stained by immunohistochemical SABC method before the distributions and morphology were observed and the numbers were counted.At last,the differences between aged and young pigs were revealed.【Result】Positive 5-HT-IR and SS-IR cells on gastric mucosa of aged and young pigs had shapes of vertebral,circle,ovoid,and spindle etc.They mainly distributed in body and bottom of gastric gland.Extremely significant differences between the numbers of the 5-HT-IR cells in gastric body and antral（P0.01）,and significant differences between gastric body and antral were observed（P0.05）.Same differences were found between gastric antral and cardiac stomach（P0.01）,while no significant difference was observed between cardiac and gastric body.There were also significant differences between the numbers of t 5-HT-IR cells and SS-IR cells in the aged and young mini pigs（P0.01）.【Conclusion】The morphologies and distribution of positive 5-HT-IR and SS-IR cells on gastric mucosa of Guizhou miniature aged pigs were similar to the youth pigs,but the secretion 5-HT and SS cells number were less,indicating that gastric endocrine function of Guizhou miniature pigs decreased as the increase of age.

关 键 词：老龄贵州小型猪 胃黏膜 5-羟色胺 生长抑素 

分 类 号：S828.89[农业科学—畜牧学]