3860例不同标本结核分枝杆菌DNA检测结果分析  

Analysis of Mycobacterium tuberculosis DNA detection results of 3860 different samples

作　　者：李锐成[1] 刘昕阳[1] 邹菊贤[1] 闫琳[1] 杨文青[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院临床实验与检验输血科,陕西西安710038

出　　处：《中国卫生检验杂志》2013年第8期1987-1989,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解实时荧光定量PCR检测TB-DNA的临床意义及不同标本类型TB-DNA检出率的差异。方法:采用实时荧光定量PCR法对患者的血清、尿液、痰液、全血、胸腹水、脑脊液、穿刺液、灌洗液进行结核菌DNA检测。结果:3860份标本的总阳性率为2.82%,血清标本的阳性率在所有标本类型中最低,仅为0.58%,穿刺液和尿液的阳性率较高,分别为10.60%和23.18%。结论:实时荧光定量TB-DNA具有快速、简便、敏感性高、特异性强的特点,适宜多种标本类型的检测,是临床上早期诊断结核菌感染的有效方法。Objective： To investigate the clinic significance of real -time fluorescent quantitative PCR in detec- tion of Mycobacterium tuberculosis DNA （ TB - DNA）, and the differential detection rate of 8 types of samples. Methods ： The samples of serum, urine, sputum, whole blood, irrigating solution, puncture fluid, cerebrospinal fluid, pleural fluid and ascites were detected for TB - DNA by real - time fluorescent quantitative PCR assay. Re- suits： Totally 3860 different samples were detected and the total positive rate was 2.82%. Serum had the lowest positive rate among the 8 types of samples, only 0.58%. Puncture fluid and urine had higher positive rate with 10.60% and 23.18% respectively. Conclusion： Real - time fluorescent quantitative PCR is fast, simple, high sensitive and specific, and suitable for detection of different samples, which is one of the effective methods for detec- tion of TB - DNA in early diagnosis of tuberculosis.

关 键 词：实时荧光定量PCR 结核分枝杆菌 临床诊断 

分 类 号：R378.911[医药卫生—病原生物学]