溃结宁膏的体外透皮实验研究  

Experimental study on in vitro transdermal permeation of Kuijiening plaster

作　　者：朱莹[1] 王燚霈[1] 张水寒[2] 

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005 [2]湖南省中医药研究院中药研究所,湖南长沙410013

出　　处：《湖南中医杂志》2013年第8期119-120,共2页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药科研计划项目(编号:2009005);湖南省长沙市科技局资助项目(编号:K0902133-31)

摘　　要：目的:观察外用溃结宁膏对皮肤的透过性,为其透皮吸收的体内研究提供实验依据,以期进一步研究溃结宁膏的药效及其作用机制。方法:以溃结宁膏中丁香酚、芍药苷为测定指标,将溃结宁膏(2.7cm×2.7cm/片)贴于健康受试者的双臂内侧,分别于2、4、8、12、16、20、24、36、48、72、120h撕下,洗脱膏层,萃取挥发油,分别采用气相法、高效液相法测定丁香酚、芍药苷的释放量。结果:溃结宁膏中丁香酚、芍药苷成分24h前释放量较快,24h后以比较恒定的速度缓慢释放,120h的累计释放率丁香酚达60.1%,芍药苷达26.6%。结论:溃结宁膏可以通过其有效成分丁香酚、芍药苷透皮,其释放量随给药时间延长而增多,从而达到起效快、疗效持久的特点。Objective：To observe the transdermal permeation of externally applied Kuijiening plaster and provide an experimental basis for the study on in vivo transdermal absorption of Kuijiening plaster, and to further investigate the therapeutic efficacy and action mechanism of Kuijiening plaster. Methods：Kuijiening piaster （2. 7 cm × 7 cm/patch） was applied to the inner sides of two arms of each healthy subject, and the plaster was peeled off at 2,4,8,12,16,20,24,36,48,72, or 120 h after being applied ;the plaster layer was e- luted, and extraction was performed on volatile oil ; the amounts of released eugenol and paeoniflorin were determined by gas chromatog- raphy and high -performance liquid chromatography. Results：Eugenol and paeoniflorin were released from Kuijiening plaster at a high rate within 24 h after Kuijiening plaster was applied and at a constantly low rate afterwards. The total amounts of released eugenol and paeoniflorin were 60. 1% and 26. 6% , respectively, within 120 h. Conelusion：Eugenol and paeoniflorin in Kuijiening plaster can perme- ate the skin, and the releases increase over time. Kuijiening plaster has the advantages of fast acting and durable effect.

关 键 词：溃结宁膏 丁香酚 芍药苷 透皮实验 

分 类 号：R283.621[医药卫生—中药学]