EFFECT OF SURFACE CHARGE OF POLYMERIC MICELLES ON IN VITRO CELLULAR UPTAKE  

作　　者：Dan-hua Zhou Jie Zhang Guan Zhang Zhi-hua Gan 

机构地区：[1]CAS Key Laboratory of Engineering Plastics, Institute of Chemistry, Chinese Academy of Sciences [2]Novelty Research Station for SCI-TECH, Beijing University of Chemical Technology [3]Department of Urology, China-Japan Friendship Hospital [4]State Key Laboratory of Organic-inorganic Composites, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology

出　　处：《Chinese Journal of Polymer Science》2013年第9期1299-1309,共11页高分子科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 51025314, 50973122,);the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KJCX2-YW-H19)

摘　　要：This work focuses on the interaction between polymeric micelles with different charged surfaces and cancer cells in order to study the influence of surface charge on the in vitro cellular uptake efficiency. The amphiphilic diblock copolymers poly（e-caprolaetone）-b-poly（ethylene oxide） （PCL-b-PEO） with different functional groups at the end of hydrophilic block were synthesized. The functional groups endue the micelles with different charges on the surfaces. The cellular uptake of micelles to T-24 cells （human bladder tumor cells）, HepG2 cells （human liver hepatocellular carcinoma cell line） and Hela cells （human epithelial cervical cancer cells） was studied by means of flow cytometer and confbcal laser scanning microscopy. The results indicate that the surface charges showed great influence on zeta potential of micelles at different pH values. The in vitro cellular uptake efficiency of micelles with different charged surfaces demonstrated different cellular uptake patterns to three kinds of cancer cells.This work focuses on the interaction between polymeric micelles with different charged surfaces and cancer cells in order to study the influence of surface charge on the in vitro cellular uptake efficiency. The amphiphilic diblock copolymers poly（e-caprolaetone）-b-poly（ethylene oxide） （PCL-b-PEO） with different functional groups at the end of hydrophilic block were synthesized. The functional groups endue the micelles with different charges on the surfaces. The cellular uptake of micelles to T-24 cells （human bladder tumor cells）, HepG2 cells （human liver hepatocellular carcinoma cell line） and Hela cells （human epithelial cervical cancer cells） was studied by means of flow cytometer and confbcal laser scanning microscopy. The results indicate that the surface charges showed great influence on zeta potential of micelles at different pH values. The in vitro cellular uptake efficiency of micelles with different charged surfaces demonstrated different cellular uptake patterns to three kinds of cancer cells.

关 键 词：Polymeric micelles Surface charge pH value Cellular uptake efficiency. 

分 类 号：O631.3[理学—高分子化学]