糖蛋白非转移性黑色素瘤蛋白B对人肝癌细胞增殖、凋亡和侵袭能力的影响及其分子机制  

Effect of GPNMB on proliferation,apoptosis and invasion of human hepatoma cells

作　　者：张承彦[1] 谢鑫[2] 张春喜[3] 高登升[4] 

机构地区：[1]陕西能源职业技术学院,陕西西安710613 [2]西北大学,陕西西安710069 [3]西安航天总医院麻醉科,陕西西安710100 [4]西安市第四医院新生儿科,陕西西安710014

出　　处：《中国病理生理杂志》2013年第8期1441-1446,共6页Chinese Journal of Pathophysiology

摘　　要：目的:研究糖蛋白非转移性黑色素瘤蛋白B(glycoprotein nonmetastatic melanoma protein B,GPNMB)对肝癌细胞增殖、凋亡和侵袭能力的影响及其分子机制。方法:以人HepG2细胞为研究对象,构建GPNMB的小干扰RNA(small interfering RNA,siRNA)以及过表达载体,转染入细胞后分别采用MTT法、流式细胞术和Transwell小室法观察其对HepG2细胞增殖、凋亡及侵袭能力的影响。结果:增殖实验结果表明GPNMB可促进HepG2细胞的增殖;流式细胞术检测发现GPNMB对HepG2细胞凋亡几乎无影响;细胞侵袭结果表明GPNMB可促进HepG2细胞的侵袭;当整合素β1亚基基因被siRNA沉默后,GPNMB对HepG2细胞增殖和侵袭的促进作用均受到明显抑制。结论:GPNMB可能通过与整合素β1亚基相互作用促进肝癌细胞的增殖和侵袭能力,提示GPNMB可以作为治疗肝癌的潜在靶点。AIM： To investigate the effect of glycoprotein nonmetastatic melanoma protein B （GPNMB） on the proliferation, apoptosis and invasion of human hepatoma HepG2 cells and its molecular mechanisms. METHODS： The GPNMB siRNA and GPNMB-overexpressing vector were constructed, and then transfected into HepG2 cells. MTT assay, flow cytometry and Transwell chamber were used to determine the effects of GPNMB down-regulation and up-regulation on the proliferation, apoptosis and invasive ability of HepG2 cells. P^SULTS： The proliferation of HepG2 cells was obviously promoted by the up-regulation of GPNMB. No effect of GPNMB on the apoptosis of HepG2 cells was observed. The invasion of HepG2 cells was also significantly promoted by the up-regulation of GPNMB. When integrin β1 was silenced by siRNA, the promoting effect of GPNMB on the proliferation and invasive ability of HepG2 cells was significantly suppressed. CONCLUSION： GPNMB may promote the proliferation and invasion ofHepG2 cells by the interaction with integrin β1, and may be used as a potential therapeutic target in liver cancer. [

关 键 词：糖蛋白非转移性黑色素瘤蛋白B HEPG2细胞 细胞增殖 细胞凋亡 肿瘤侵袭 

分 类 号：R395.2[哲学宗教—心理学]