神经元核心抗原和神经元特异性烯醇化酶在人胚胎小肠发育阶段的表达  

Expression of neuronal nuclear antigen and neuron-specific enolase in developing human embryonic small intestines

作　　者：刘学红[1] 张泳[1] 

机构地区：[1]绍兴文理学院医学院,浙江绍兴312000

出　　处：《中国病理生理杂志》2013年第8期1512-1514,1524,共4页Chinese Journal of Pathophysiology

基　　金：浙江省教育厅科研计划项目(No.Y201018535)

摘　　要：目的：探讨神经元核心抗原（neuronal nuclear antigen，NeuN）和神经元特异性烯醇化酶（neuron-spe-dificenolase，NSE）在人胚胎小肠发育阶段的分布规律及其表达意义。方法：应用免疫组织化学法检测第2—4月龄段共16例人胚胎小肠壁细胞NeuN和NSE的表达、分布状况。结果：第2—4月胎龄段，NSE在人胚胎小肠肌间神经丛内的神经元及神经纤维均呈强阳性表达，在黏膜下层，随着胎龄的增大，NSE阳性表达细胞和纤维数量逐渐增多，在肠腺内均有少量散在分布的NSE阳性细胞；在黏膜层的腺体和上皮组织内均有散在的NeuN阳性细胞分布，随着胎龄的增大，NeuN阳性细胞数量增多；在肌间神经丛，第3月龄段开始，有少量NeuN阳性细胞，随着胎龄的增大，NeuN阳性细胞数量逐渐增多；在黏膜下层，未见NeuN阳性细胞分布。结论：人胚胎小肠发育阶段，NeuN和NSE在小肠壁的阳性表达和分布不一致，均参与小肠壁神经元及神经内分泌细胞的发育过程。AIM： To investigate the neuronal nuclear antigen （NeuN） and neuron-specific enolase （NSE） distribution in the developing stage of human embryonic small intestines and their clinical significance. METHODS： Six-teen cases of human embryos at 2 - 4 months of gestational age were used in the present study. The technique of immunohistochemical staining was used to detect the expression and distribution of NeuN and NSE in small intestinal walls. RE- SULTS ： At 2 ~ 4 months of gestational age, NSE was strongly expressed in neurons and nerve fibers of the small intestinal myenteric nerve plexuses in human embryos. The numbers of NSE-pesitive cells and fibers gradually increased in the small intestinal submucosa with the increase in gestational age, and a few NSE-positive cells located in the small intestinal glands of human embryos. NeuN-peSitive cells scattered in the epithelium and glands of the small intestinal mucosa, and the num- ber of NeuN-positive cells gradually increased with the increase in gestational age. But there were no NeuN-positive cells in the small intestinal submucosa. CONCLUSION： The expression and distribution of NeuN are not consistent with those of NSE during the development of human embryonic small intestines, and beth of them may be involved in the development of neurons and neuroendocrine ceils in small intestinal walls. [

关 键 词：小肠 神经元核心抗原 神经元特异性烯醇化酶 胚胎发育 神经元 神经纤维 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学]