电针对大鼠吗啡耐受时脊髓背角细胞外信号调节激酶1／2活性的影响  

Effect of electroacupuncture on activity of extracellular signal-regulated kinase 1/2 in spinal dorsal horn during morphine tolerance in rats

作　　者：郑宇欣[1] 于泳浩[1] 柴韵[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2013年第5期554-557,共4页Chinese Journal of Anesthesiology

基　　金：天津市卫生局科技基金（2010KZ107）

摘　　要：目的探讨电针对大鼠吗啡耐受时脊髓背角细胞外信号调节激酶1／2（ERK1／2）活性的影响。方法取鞘内置管成功的雄性sD大鼠25只，采用随机数字表法，将其分为5组（n=5）：生理盐水组（Ns组）鞘内注射生理盐水10μl；吗啡组（M组）鞘内注射吗啡10μg；错义寡核苷酸组（MO组）鞘内注射吗啡10μg＋ERK1／2错义寡核苷酸10μg；反义寡核苷酸组（AO组）鞘内注射吗啡10μg＋ERK1／2反义寡核苷酸10μg；电针组（EA组）鞘内注射吗啡10μg，同时每日首次给药后电针大鼠阳陵泉和足三里（频率2Hz，波宽1ms，电流强度3mA，刺激时间30min）。各组注射药物容量均为10μl，2次，d，连续7d。于鞘内给药前、鞘内给药2、4、6d和鞘内给药结束后1d（T0-4）测定机械痛阈。于T4时机械痛阈测定结束后，取脊髓背角组织，采用Western blot法测定大鼠ERK1／2和磷酸化ERK1／2（p-ERK1／2）的表达。结果M组、MO组和AO组和EA组发生了吗啡耐受，EA组吗啡耐受程度最轻。与Ns组比较，M组和MO组p-ERK1／2表达上调，AO组总ERK1／2表达下调（P〈0．05），EA组上述指标差异无统计学意义（P〉0．05）；与M组和MO组比较，AO组p-ERK1／2和总ERK1／2表达下调，EA组p-ERK1／2表达下调（P〈0．05）；与AO组比较，EA组p-ERK1／2和总ERK1／2表达上调（P〈0．05）。结论电针可抑制慢性吗啡给药所导致的脊髓背角ERKI／2活性升高，从而缓解吗啡耐受的形成。Objective To investigate the effect of electroacupuncture （EA） on the activity of extracellular signal-regulated kinase 1/2 （ERK1/2） in the spinal dorsal horn during morphine tolerance in rats. Methods Twenty-five healthy male Sprague-Dawley rats in which intrathecal （IT） catheters were successfully implanted with- out complications were randomly divided into 5 groups （ n = 5 each） ： group normal saline （NS） 10 /~1 IT twice a day x 7 days; group M morphine 10μg （10 μl） IT twice a day x 7 days; group MO morphine 10/lg ＋ missense oligonucleotide （ODN） 10μg （10μl） twice a day x 7 days; group AO morphine 10μg ＋ antisense ODN 10 μg （10/A） twice a dayx7 days; group EA morphine 10μg （10 μl） twice a dayx7 days ＋ EA （frequency 2 Hz, wave length 1 ms, intensity 3 mA）. EA of Yanglingquan and Zusanli lasting for 30 min was performed after first IT administration of morphine once a day for 7 days. Mechanical pain threshold （MPT） was measured before IT ad- ministration and at day 2, 4, 6 of IT administration and 1 day after the end of IT administration. The animals were sacrificed after the last MPT measurement, and L3_5 segments of the spinal cord were isolated to detect the expres- sion of ERK1/2 and phosphor-ERK1/2 （p-ERK1/2） in the spinal dorsal horn by Western blot analysis. Results Morphine tolerance developed in groups M, MO and AO, and was lightest in group EA. Compared with group NS, the expression of p-ERK1/2 was significantly up-regulated in groups M and MO, the expression of ERK1/2 was down-regulated in group AO （P 〈 0.05）, and no significant change was found in the indexes mentioned above in group EA （P 〉 0.05）. Compared with groups M and MO, the expression of p-ERK1/2 and ERK1/2 was significantly down-regulated in group AO, and the expression of p-ERKI/2 was down-regulated in group EA （ P 〈 0.05 ）. Compared with group AO, the expression of p-ERK1/2 and ERK1/2 was significantly up-regulated in group EA （ P 〈 0.05）. Conclusi

关 键 词：细胞外信号调节MAP激酶类 电针 药物耐受性 吗啡 

分 类 号：R24[医药卫生—中医临床基础]