参附汤预先给药对内毒素血症大鼠肠黏膜屏障功能的影响  

Effect of Shenfu Decoction pretreatment on function of intestinal mucosal barrier of endotoxemic rats

作　　者：罗福全[1] 李大珍[1] 叶茂[1] 徐颖[1] 刘巍[1] 李义辉[1] 

机构地区：[1]重庆医科大学附属儿童医院麻醉科儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地,400014

出　　处：《中华麻醉学杂志》2013年第5期609-611,共3页Chinese Journal of Anesthesiology

基　　金：重庆市卫生局中医药科研计划项目（2008-2-53）

摘　　要：目的评价参附汤预先给药对内毒素血症大鼠肠黏膜屏障功能的影响。方法选择健康成年雄性sD大鼠24只，4～6月龄，体重180～220g。采用随机数字表法，将其分为3组（n=8）：正常对照组（C组）、内毒素血症组（E组）和参附汤预先给药组（S组）。采用尾静脉注射脂多糖（LPS）10mg／kg建立内毒素血症模型。建模前30min，E组和s组分别腹腔注射生理盐水和参附汤5ml／kg。于建模后2h时取腹主动脉血样，采用ELISA法检测血浆TNF-α、IL-6、肠-脂肪酸结合蛋白（i-FABP）和D-乳酸（D．1ac）浓度；取远端回肠，采用免疫组化法检测肠黏膜上皮细胞间紧密连接蛋白（ZO-1）表达，HE染色，光镜下观察肠黏膜病理学结果，并行Chiu评分。结果与c组比较，E组和S组血浆TNF-α、IL-6、i-FABP和D-lac浓度升高，肠黏膜Chiu评分升高，肠黏膜上皮细胞间ZO-1表达下调（P〈0．05）。与E组比较，s组血浆TNF—α、IL-6、i-FABP和D．1ac浓度降低，肠黏膜Chiu评分降低，肠黏膜上皮细胞间ZO-1表达上调（P〈0．05）。结论参附汤预先给药可减轻内毒素血症大鼠肠黏膜损伤，保护肠黏膜屏障功能，其机制与抑制全身炎性反应有关。Objective To evaluate the effect of Shenfu Decoction pretreatment on the function of intestinal mucosal barrier of endotoxemic rats. Methods Twenty-four adult male Sprague-Dawley rats, aged 4-6 months, weighing 180-220 g, were randomly divided into 3 groups （n = 8 each） ： blank control group （group C）, endotoxemia group （group E） and Shenfu Decoction pretreatment group （group S）. Endotoxemia was induced by lipopo- lysaceharide 10 mg/kg injected intravenously in anesthetized rats in groups E and S. Normal saline and Shenfu De- coction 5 ml/kg were injected intraperitoneally at 30 rain before the model of endotoxemia was established in groups E and S, respeetively. At 2 h after the model of endotoxemia was established, blood samples were obtained from the abdominal aorta for measurement of the plasma concentrations of tumor necrosis factor-α （TNF-a）, interleukin- 6 （IL-6）, intestinal fatty acid binding protein （i-FABP） and diamine oxidase （DAO） by ELISA. The distal ileum was harvested for detection of the expression of tight junctional protein Zonula Occludens-1 （ZO-1 ） in the intestinal mucosa of epithelial cells by immunohistochemistry. The changes in pathological morphology of the intestinal muco- sa were examined after one segment of the distal ileum was stained with haematoxylin and eosin. The pathological changes were assessed and graded according to Chiu＇s score. Results Compared with group C, the plasma TNF-α, IL-6, i-FABP and D-lac concentrations were significantly increased, Chiu＇ s scores were increased, and the expression of ZO-1 was down-regulated in groups E and S （P 〈 0.05） . Compared with group E, the plasma TNF-α and IL-6 concentrations were significantly decreased, the plasma i-FABP and D-lac concentrations were decreased, Chiu＇ s scores were decreased, and the expression of ZO- 1 was up-regulated in group S （ P 〈 0.05 ）. Conclusion Shenfu Decoction pretreatmcnt can attenuate the damage to the intestinal mucosa and protect the function of

关 键 词：参附汤 内毒素血症 肠黏膜 

分 类 号：R285.5[医药卫生—中药学]