哺乳期大鼠反复七氟醚麻醉对远期认知功能的影响  

Effects of repeated sevoflurane anesthesia on long-term cognitive function in lactating rats

作　　者：郑淑文[1] 朱昭琼[1] 马欢欢[1] 张超[1] 周军[1] 

机构地区：[1]遵义医学院附属医院麻醉科,563003

出　　处：《中华麻醉学杂志》2013年第6期669-671,共3页Chinese Journal of Anesthesiology

基　　金：贵州省科技厅重点资助项目（黔科合J字^[2007]2132）;吸入麻醉特助项目（遵鲁药^[2010]001号）

摘　　要：目的评价哺乳期大鼠反复七氟醚麻醉对远期认知功能的影响。方法健康7d龄sD大鼠24只，体重14。17g，采用随机数字表法，将其分为3组（n=8），对照组（c组）、2．6％七氟醚组（s1组）、1．5％七氟醚组（s2组）。于出生7、14、21d时吸入2．6％七氟醚（s，组）、1．5％七氟醚（S2组）或运载气体（c组）1h，于出生28、29d时行可视平台实验，记录游泳速度；于出生32—36d时行定位航行实验，记录逃避潜伏期；于出生36d时行空间探索实验，记录平台区域滞留时间、游泳总路程及穿越原平台次数。结果与c组比较，S1组和S2组逃避潜伏期延长（P〈0．05）。与S2组比较，S1组逃避潜伏期延长（P〈0．05）。3组游泳速度、平台区域滞留时间、游泳总路程及穿越原平台次数比较差异无统计学意义（P〈0．05）。结论哺乳期大鼠反复七氟醚麻醉可损害远期陈述性记忆能力，并与吸入浓度有关，而对联合型学习能力无影响。Objective To evaluate the effects of repeated sevoflurane anesthesia on long-term cognitive function in lactating rats. Methods Twenty-four healthy Sprague-Dawley rats, aged 7 days, weighing 14-17 g, were randomly divided into 3 groups （ n = 8 each） ： control group （ group C）, 2.6 % sevoflurane group （ group S1 ）, and 1.5% sevoflurane group （group S2） ~ At 7, 14 and 21 days after birth, 2.6% and 1.5% sevoflurane and carrier gas were inhaled for 1 h in groups SI , S2 and C, respectively. Visible platform trial was carried out on 28 and 29 days after birth, and the swimming speed of the rats was recorded. Place navigation test was performed on 32-36 days after birth, and the escape latency was recorded. Spatial probe test was carried out on 36 days after birth, and the time spent in tho. platform quadrant, swimming distance and the number of times crossing the platform quadrant was recorded. Results Compared with group C, the escape latency was significantly prolonged in groups S1 and S2 （ P 〈 0.05）. The escape latency was significantly longer in group St than in group S2 （ P 〈 0.05）. There were no signifcant differences between the three groups in the swimming speed, time spent in the platform zone, swimming distance and the number of times crossing the platform quadrant （ P 〉 0.05 ）. Conclusion Repeated inhalation of sevoflurane during lactation can impair the long-term declarative memory, which is concentration-related, while it has no effects on the associative learning in rats.

关 键 词：婴儿 认知障碍 儿童 七氟醚 

分 类 号：R614[医药卫生—麻醉学]