伤害性刺激因素对丙泊酚麻醉时犬脊髓γ-氨基丁酸分布的影响  

Effect of noxious stimulation factor on γ-aminobutyric acid distribution in dog spinal cord during propofol anesthesia

作　　者：吉锦泉[1] 赵国栋[1] 徐金东[1] 周国斌[1] 

机构地区：[1]广东省人民医院(广东省医学科学院)麻醉科,广州市510080

出　　处：《中华麻醉学杂志》2013年第6期694-696,共3页Chinese Journal of Anesthesiology

基　　金：广东省科技计划项目（2011B031800149）

摘　　要：目的评价伤害性刺激因素对丙泊酚麻醉时犬脊髓γ-氨基丁酸（GABA）分布的影响。方法12～18月龄健康杂种犬16只，雌雄不拘，体重10，12kg，采用随机数字表法，将其分为2组（n=8）：伤害性刺激组（s组）和对照组（c组）。静脉注射丙泊酚7mg/kg进行麻醉诱导，插入气管导管，连接麻醉机行机械通气，右股动脉穿刺置管监测MAP和脉率，静脉输注丙泊酚70mg-kg^-1·h^-1维持麻醉。s组于犬尾中部皮下注射5％福尔马林300出，C组注射等容量生理盐水。于注射福尔马林（或生理盐水）前（T1）和注射福尔马林（或生理盐水）后（T7）记录MAP和脉率。持续静脉输注丙泊酚50min时处死动物，取颈椎2,3节段脊髓组织，采用高效液相色谱法，分别检测脊髓不同区域（前角、背角、中间带、前索、后索、外侧索）GABA水平。结果与，T1时相比，T2时s组MAP和脉率均升高（P〈0．05）。C组脊髓不同区域（前角、背角、中间带、前索、后索、外侧索）GABA水平比较差异无统计学意义（P〉0．05）。与c组相比，s组脊髓前角和背角GABA水平升高（P〈0．05），其它区域GABA水平差异无统计学意义（P〉0．05）。结论伤害性刺激因素可引起丙泊酚麻醉时犬脊髓前角和背角GABA水平升高。Objective To evaluate the effect of the noxious stimulation factor on γ-aminobutyric acid （GABA） distribution in dog spinal cord during propofol anesthesia. Methods Sixteen healthy mongrel dogs of both sexes, aged 12-18 months, weighing 10-12 kg, were randomly divided into 2 groups （ n = 8 each） ： noxious stimulation group （S group） and control group （C group）. Anesthesia was induced with propofol 7 mg/kg. The animals were mechanically ventilated after tracheal intuhation. Right femoral artery was cannulated for mean arterial pressure （MAP） and pulse rate monitoring. Anesthesia was maintained with propofol infusion at a constant rate of 70 mg·kg-1· h-1 . 5 % formalin 300 μl was subcutaneously injected into the central region of tails in group S, while the equal volume of normal saline was injected instead of formalin in group C. MAP and pulse rate were recorded before injection of formalin or normal saline （T1） and after injection of formalin or normal saline （T2）. The dogs were scarified by decapitation at 50 min of continuous propofol infusion and cervical 2-3 segments of the spinal cord were removed for determination of GABA level in different regions of the spinal cord （frontal horn, posterior horn, intermediate zone, frontal funiculus, posterior funiculus and lateral funiculus） by HPLC. Results MAP and pulse rate were significantly higher at T2 than at % in S group （ P 〈 0.05 ）. There were no significant differences in GABA level among the different regions of the spinal cord in C group （ P 〉 0.05） . Compared with C group, GABA level in the frontal horn and posterior horn was significantly increased （P 〈 0.05 ）, and no significant change was found in the other regions of the spinal cord in S group （ P 〉 0.05）. Conclusion The noxious stimulation factor can induce an increase in GABA level in the frontal horn and posterior horn of dog spinal cord during propofol anesthesia.

关 键 词：刺激 化学性 二异丙酚 脊髓 受体 GABA 

分 类 号：R614[医药卫生—麻醉学]