切口痛大鼠胃体胃动素水平的变化  

Changes in levels of motilin in gastric body in a rat model of incisional pain

作　　者：张瑜[1] 赵军[2] 高宝柱[2] 郑宝森[3] 曹君利[4] 

机构地区：[1]天津医科大学附属肿瘤医院麻醉科,300060 [2]天津市第三中心医院麻醉科 [3]天津医科大学第二医院疼痛治疗中心 [4]徐州医学院江苏省麻醉与镇痛应用技术重点实验室

出　　处：《中华麻醉学杂志》2013年第6期697-700,共4页Chinese Journal of Anesthesiology

基　　金：天津市卫生局科技基金（2010KZ19）

摘　　要：目的探讨切口痛大鼠胃体胃动素水平的变化。方法SPF级健康雄性sD大鼠84只，6～8周龄，体重180～220g，采用随机数字表法，将其分为2组（n=42）：生理盐水组（Ns组）和切口痛组（P组）。P组行右足跖肌切口以制备切口病模型。2组随机取6只大鼠，于术前24h（To）、术后1h（T1）、6h（T2）、24h（T1）、48h（T4）、72h（T5）测定机械缩足反应阈（MWT）与热缩足反应潜伏期（TWL），并于上述各时点分别随机取6只大鼠，断头处死，均取胃体粘膜组织，采用ELISA法测定胃动素水平。结果与Ns组比较，P组T1～T4时MWT、TWL和胃体胃动素水平降低（P〈0．05），rrn、B时差异无统计学意义（P〉0．05）；与L时比较，P组MWT、TwL和胃体胃动素水平T1～T4时降低，L时差异无统计学意义（P〉0．05）；与T1时比较，P组MWT、TWL和胃体胃动素水平T3～T5时升高（P〈0．05）；P组MWT、TWL和胃体胃动素水平T3～T5时逐渐升高（P〈0．05）。P组胃体胃动素水平与MWT、TWL均呈正相关性（r分别为0．9597、0．9231，P〈0．01）。结论切口痛大鼠胃体胃动素水平明显降低，降低幅度与切口痛程度呈正相关。Objective To investigate the changes in the levels of motilin in the gastric body in a rat model of incisional pain.Methods Eighty-four healthy male Sprague-Dawley rats, weighing 180-220 g, were randomized into 2 groups （ n = 42 each） ： normal saline group （NS group） and incisional pain group （P group）. In group P an 1 cm long incision was made in the plantar surface of right hindpaw under sevoflurane anesthesia. Six rats were chosen from each group and paw withdrawal threshold to mechanical stimulation （MWT） and paw withdrawal laten- cy to nociceptive thermal stimulation （TWL） were measured at 24 h before operation （To ） and 1, 6, 24, 48 and 72 h after operation （T1-5）. Six rats were chosen from each group at T0-5 and sacrificed and the gastric mucosal tissues were prepared for measurement of motilin levels by ELISA. Results Compared with group NS, MWT, TWL and motilin levels were significantly decreased at T1-4 （ P 〈 0.05） and no significant change was found at TO and T5 in P group （P 〉 0. 05）. Compared with the baseline value at To , MWT, TWL and motilin levels were significantly decreased at T1-4 , and no significant change was found at T5 in group P （ P 〉 0.05）. MWT,TWL and motilin levels were significantly higher at T3 -T5 than at TI in group P （ P 〈 0.05）. MWT, TWL and motilin levels were increased gradually at T3-T5 in P group （ P 〈 0.05）. The motilin levels were positively correlated with MWT and TWL （ r = 0. 9597 and 0.9231, respectively, P 〈0. 01 ） in group P. Conclusion The levels of motilin in the gastric body is significantly decreased in a rat model of incisional pain and the decreasing range is positively correlated with the degree of incisional pain.

关 键 词：疼痛 手术后 促胃动素 胃 

分 类 号：R6[医药卫生—外科学]