大鼠窒息性心脏停搏复苏后心肌微小RNA表达的变化  

Dynamic changes in myocardial microRNA expression after cardiopulmonary resuscitation in a rat model of asphyxial cardiac arrest

作　　者：何爱文[1] 刘玉荣[1] 江哲龙 陈寿权 李章平[1] 李冬芳[1] 乔江华[1] 李惠萍[1] 黄唯佳[1] 程俊彦[1] 

机构地区：[1]温州医科大学附属第一医院急诊科,325000 [2]本大学附属第二医院急诊科

出　　处：《中华麻醉学杂志》2013年第6期729-732,共4页Chinese Journal of Anesthesiology

基　　金：温州市科技计划项目（Y20090241,Y20080084）

摘　　要：目的探讨大鼠窒息性心脏停搏复苏（CA-CPR）后心肌微小RNA（miRNA）表达的动态变化。方法清洁级成年雄性sD大鼠64只，体重250～330g，采用随机数字表法，将其分为2组（n=32），对照组（C组）和CA-CPR组。采用窒息法建立CA—CPR模型。CA-CPR组于自主循环恢复后3、6、12和24h（T1）时，c组于相应时点取血样，测定血清CK—MB和cTnI浓度，相应时点取心尖部心肌组织，采用Real—TimePCR法检测心肌组织miRNA。以相对于C组心肌miRNA2倍强度的变化作为CA—CPR组miRNA表达上调或下调的标准。记录大鼠均发生表达变化的miRNA。结果与C组比较，CA—CPR组各时点血清CK．MB和cTnl浓度升高（P〈0．01）；CA—CPR组血清CK—MB浓度T1时达峰值，血清cTnI浓度持续升高（P〈0．05或P〈0．01）。自主循环恢复后持续性变化的miRNA有8个，分别为miRNA-1、miRNA-21、miRNA126、miRNA．133a、miRNA，206、miRNA-210、miRNA-320及miRNA-499。miRNA-1各时点表达均上调，T2时最高；miRNA-21各时点表达均下调，T2时最低；miRNA126、miRNA-133a、miRNA-206、miRNA-210和miRNA．499T1、T2时表达下调，T2时最低，T3、T4时表达上调；miRNA．320T1～T3时表达上调，T2时最高，T4时表达下调（P〈0．05或0．01）。结论心脏停搏大鼠复苏后有8个miRNA表达发生变化，且变化方式（上调／下调）、趋势不同，但表达变化方式改变的时点相似。Objective To investigate the dynamic changes in myocardial microRNA （miRNA） expression after cardiopulmonary resuscitation in a rat model of asphyxial cardiac arrest. Methods Sixty-four adult male Sprague-Dawley rats, weighing 250-330 g, were randomly divided into 2 groups （ n = 32 each） ： control group （group C） and cardiopulmonary resuscitation after cardiac arrest group （group CA-CPR）. The rats were anesthetized with intraperitoneal chloral hydrate 7 ml/kg, tracheostomized and mechanically ventilated. The model of cardiac arrest was established by clamping the endotracheal tube at end expiration and cardiopulmonary resuscitation was per- formed. At 3, 6, 12 and 24 h after recovery of spontaneous circulation （T1_4）, blood samples were collected for determination of serum creatine-kinase isoenzyme MB （CK-MB） and cardiac troponin I （cTnI） concentrations and myocardial specimens were obtained from the apex for detection of the expression of miRNAs in myocardial tissues （by using RT-PCR）.Results Compared with group C, serum CK-MB and cTnI concentrations were significantly increased at each time point in group CA-CPR （P 〈 0.01 ）. The serum CK-MB concentration reached the peak at T3 , and the serum cTnI concentrations continued to increase in group CA-CPR （ P 〈 0.05 or 0.01 ）. There were 8 miRNAs which changed continuously after recovery of spontaneous circulation, and there were miRNA-1, miRNA-21, miRNA126, miRNA-133a, miRNA-206, miRNA-210, miRNA-320 and miRNA-499. The expression of miRNA-1 was up-regulated at each time point and peaked at T2 · The expression of miRNA-21 was down-regulated at each time point and reached the lowest level at T2 · The expression of miRNA126, miRNA-133a, miRNA-206, miRNA-210 and miRNA-499 was down-regulated at T1 and T2 , was up-regulated at T3 and T4, and reached the lowest level at T2. The expression of miRNA-320 was up-regulated at T1-T3, was down-regulated at T4, and peaked at T2 （ P 〈 0.05 or 0.01 ）. Conclusion Eight miRNAs exhi

关 键 词：心脏停搏 心肺复苏术 微小RNA 

分 类 号：R541[医药卫生—心血管疾病]