不同阿片受体在舒芬太尼后处理减轻大鼠心肌缺血再灌注损伤中的作用  

Role of different opioid receptors in sufentanil postconditioning-induced attenuation of myocardial ischemiareperfusion injury in rats

作　　者：曹袁媛[1] 顾尔伟[1] 方卫平[1] 陈巧玲[1] 朱燕[1] 张雷[1] 

机构地区：[1]安徽医科大学第一附属医院麻醉科,合肥市230022

出　　处：《中华麻醉学杂志》2013年第6期742-745,共4页Chinese Journal of Anesthesiology

基　　金：安徽省教育厅重点科研项日（KJ2010A180）;安徽高校省级自然科学研究项目（KJ20122144）

摘　　要：目的探讨不同阿片受体在舒芬太尼后处理减轻大鼠心肌缺血再灌注损伤中的作用。方法健康雄性sD大鼠50只，4～6月龄，体重200～330g，采用随机数字表法，将其分为9组，缺血再灌注组（I／R组，n=7）采用结扎左冠状动脉前降支（LAD）30min、再灌注120min的方法制备大鼠心肌缺血再灌注损伤模型；缺血后处理组（IPC组，n=7）再灌注前行缺血后处理，进行3个循环的10s开放LAD10s阻断LAD；舒芬太尼后处理组（sP组，n=6）于再灌注前5min静脉注射舒芬太尼1μg／kg行舒芬太尼后处理；x受体拮抗剂nor-binaltorphimne＋舒芬太尼后处理组（BNI＋SP组，n=5）、8受体拮抗剂纳曲吲哚＋舒芬太尼后处理组（NTD＋SP组，n=5）和μ受体拮抗剂CTOP＋舒芬太尼后处理组（CTOP＋sP组，n=5）于舒芬太尼后处理前分别静脉注射BNI5mg／kg、纳曲吲哚5mg／kg和CTOP1mg／kg；BNI组、NTD组和CTOP组分别于再灌注前5min时静脉注射相应剂量BNI、纳曲吲哚和CTOP。于再灌注120min时采集颈总动脉血样，测定血浆cTnI浓度，处死大鼠后，取心脏，确定心肌梗死（Is）与缺血危险区（AAR）体积的比值（IS／AAR值）。结果与I／R组比较，IPC组、sP组、BNI＋sP组和NTD＋sP组血浆cTnI浓度和IS／AAR值降低（P〈0．05），CTOP＋sP组、NTD组、BNI组和CTOP组血浆cTnI浓度和IS／AAR值差异无统计学意义（P〉0．05）；与sP组比较，BNI＋SP组和NTD＋sP组IS／AAR值升高，CTOP＋sP组血浆cTnI浓度和IS／AAR值升高（P〈0．05）。结论队＊和6阿片受体均介导了舒芬太尼后处理减轻大鼠心肌缺血再灌注损伤。Objective To evaluate the role of different opioid receptors in sufentanil postconditioning-induced attenuation of myocardial ischemia-repeffusion （I/R） injury in rats. Methods Fifty adult male Sprague- Dawley rats, aged 4-6 months, weighing 200-330 g, were randomly divided into 9 groups： I/R group （ n = 7）, ischemic postconditioning （IPC） group （ n = 7）, sufentanil postconditiong （SP） group （ n = 6）, κ-opioid receptor antagonist nor-binaltorphimine （nor-BNI） ＋ SP group （group BNI ＋ SP, n = 5） , δ-opioid receptor antagonist nal- trindole ＋ SP group （group NTD ＋ SP, n = 5） and μ-opioid receptor antagonist CTOP ＋ SP group （group CTOP ＋ SP, n = 5）. Myocardial I/R was produced by 30 min occlusion of left anterior descending branch of coronary artery followed by 120 min reperfusion. IPC was induced by 3 episodes of 10 s reperfusion followed by 10 s isehemia at the end of 30 min myocardial ischemia. Sufentanil 1 μg/kg was injected intravenously at 5 min before reperfusion in SP group. In groups BNI ＋ SP, NTD ＋ SP and CTOP ＋ SP, nor-BNI 5 mg/kg, naltrindole 5 mg/kg and CTOP 1 mg/kg were injected intravenously, respectively, before SP and the corresponding doses of nor-BNI, nahrindole and CTOP were injected intravenously, respectively, at 5 min before reperfusion. Arterial blood samples were collected at 120 min of reperfusion for measurement of the plasma cardiac tropnin I （cTnI） concentration. The rats were then sacrificed and hearts removed for determination of myocardial infarct size （IS） and area at risk （AAR）. IS/AAR was calculated. Results Compared with I/R group, the plasma cTnI concentration and IS/AAR were sig- nificantly decreased in groups IPC, SP, BNI ＋ SP and NTD ＋ SP （ P 〈 0.05 ）, and no significant change was found in the plasma cTnI concentration and IS/AAR in groups CTOP ＋ SP, NTD, BNI and CTOP （P 〉 0.05）. Compared with SP group, IS/AAR was increased in NTD ＋ SP and BNI ＋ SP groups and the plasma cTnI conce

关 键 词：舒芬太尼 受体 阿片样 心肌再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]