异养硝化-好氧反硝化菌HN-02的筛选及其特性  

Screening and Characteristics of Heterotrophic Nitrification - Aerobic Denitrification Strain HN-02

作　　者：陈茂霞[1] 王欢[2] 周后珍[1] 李杨[1] 谭周亮[1] 李旭东[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]四川省环境保护科学研究院,成都610041

出　　处：《应用与环境生物学报》2013年第4期688-693,共6页Chinese Journal of Applied and Environmental Biology

基　　金：科技部国际科技合作与交流专项(2013DFG92600);国家自然科学基金项目(51208489);四川省科技厅项目(2011SZ0303;2012SZ0161)资助~~

摘　　要：为了向生物强化处理高浓度含氮废水提供高效菌株,从活性污泥中筛选到一株效果较好的异养硝化-好氧反硝化菌,命名为HN-02.形态观察、生理生化鉴定及16S rDNA序列分析表明,该菌株为短杆状革兰氏阴性菌,并鉴定为气单胞菌属(Aeromonas),且能扩增出相关好氧反硝化基因napA.其最佳碳源为琥珀酸,其次是蔗糖和柠檬酸钠;最佳生长温度为30℃;最佳pH范围为6-9.在好氧条件下,该菌能独立降解多种形态的氮,以氨氮、亚硝氮、硝氮为唯一氮源时,24 h总氮去除率分别为77.71%、53.34%、56.80%.经定性验证,脱氮气体产物含有氮氧化物.In this study for bioaugmentation in the treatment of high nitrogen content wastewater, a well functioning heterotrophic nitrification-aerobic denitrification bacteria strain was isolated from activated sludge and named HN-02. By morphological, physiological, biochemical identification and 16S rDNA sequence analysis, the short rod-shaped Gram-negative bacteria strain was identified as Aeromonas bacteria. It＇s partial napA gene was cloned. The best carbon source for HN-02 bacteria was succinic acid, followed by sucrose and sodium citrate; the optimum temperature was 30℃C; and the optimum pH range was 6 to 9. Under aerobic conditions, various forms of nitrogen could be removed by HN-02 independently. The 24 h TN removal efficiencies were 77.71%, 53.34%, 56.80%, respectively, with ammonia nitrogen, nitrite, nitrate as sole nitrogen source. By qualitative verification, the nitrogen removal product gas contained nitrogen oxides. Fig 9, Tab 1, Ref28

关 键 词：异养硝化-好氧反硝化 菌株生长特性 脱氮性能 生物强化 气单胞菌属 

分 类 号：X703[环境科学与工程—环境工程]