多孔纳米氧化铜修饰电极的制备及分析应用(英文)  

Fabrication of nanoporous copper oxide film biosensor with a polymer sphere template and its application to glucose sensing

作　　者：刘皓[1] 钱程[1] 徐海红[1] 吴守国[1] 

机构地区：[1]中国科学技术大学化学系,安徽合肥230026

出　　处：《中国科学技术大学学报》2013年第8期645-653,共9页JUSTC

基　　金：Supported by the National Key Basic Research(973)Program of China(2013CB933900)

摘　　要：利用胶体晶体模板法和循环伏安电镀法成功地在铜电极表面合成了多孔纳米氧化铜薄膜,并应用于碱性溶液中葡萄糖的直接电催化氧化.制备的修饰电极在5μmol/L～3.2mmol/L的浓度范围内对葡萄糖具有线性响应关系,灵敏度为37.99A·m·mol-1,达到稳定电流值所需要的时间小于3s.在3倍信噪比条件下,该传感器的检测限为0.37μmol/L,明显优于其他非酶传感器,这要归功于多孔氧化铜的结构,该结构能够增加电极表面的活性位点,提高葡萄糖氧化过程中的电子转移速率.同时,该传感器对实际血样的测定具有很高的准确度,结果表明,该多孔氧化铜材料具有较高的灵敏度和选择性,在非酶葡萄糖传感器领域具有广阔的应用前景.A nanoporous CuO film biosensor was fabricated on a Cu substrate electrode by the colloidal-crystal polystyrene template technique with the help of potentiostatic plating and potential cyclic scanning. The direct electrocatalytic oxidation of glucose in alkaline medium on nanoparticle CuO film modified electrode has been investigated in detail. When applied to detect glucose, the sensor exhibited a linear response of up to 3.2 mmol/L of glucose, and a high sensitivity of 3 799μA·mM^-1·cm^-2, higher than that of most nonenzymatic glucose sensors reported in the literature. The sensor response time was less than 3 s and the detection limit was 0. 37 μmol/L （at signal/noise=3）. This improvement is due to the nanoporous structure and the greatly enhanced effective surface area. In addition, the sensor also performed well for measuring glucose concentrations in human blood serum samples. The results indicate that the nanoporous CuO film modified electrode allows highly sensitive and selective, stable, and fast amperometric sensing of glucose and is thus promising for the further application in nonenzymatic glucose sensors development.

关 键 词：聚苯乙烯模板 电沉积 葡萄糖 非酶生物传感器 

分 类 号：O657[理学—分析化学]