HPLC测定不同产地土茯苓中落新妇苷和黄杞苷含量  

HPLC determination of astilbin and engeletin in Smilax glabra from different producing areas

作　　者：白梅[1] 刘为萍[1] 李素珍[1] 

机构地区：[1]桂林医学院附属医院临床医学实验教学中心,桂林541001

出　　处：《药物分析杂志》2013年第8期1352-1354,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用HPLC法对不同产地的土茯苓中落新妇苷和黄杞苷的含量进行测定。方法:采用Waters SUNfire ODS(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水-磷酸溶液(35∶65∶0.1)为流动相,流速1.0 mL.min-1,柱温35℃,检测波长为290 nm。结果:落新妇苷和黄杞苷分别在0.051～1.02μg和0.058～1.16μg范围内内呈良好的线性关系,平均加样回收率(n=9)为99.23%和99.93%。结论:本方法可用于土茯苓药材的质量控制;不同产地土茯苓中落新妇苷和黄杞苷的含量差异较大。Objective： To determine astilbin and engeletin in Smilax glabra from different producing areas by HPLC.Methods： The samples were separated at 35 ℃ on a Waters SUNfire ODS column（4.6 mm × 250 mm,5 μm） eluted with methanol-water-phosphoric acid（35∶ 65∶ 0.1） as the mobile phase.The flow rate was at 1.0 mL.min-1 and the detection wavelength was 290 nm.Results： The calibration curves of astilbin and engeletin were linear over the ranges of 0.051-1.02 μg and 0.058-1.16 μg,the average recoveries（n = 9） were 99.23% and 99.93%,respectively.Conclusion： The method can be used as a quality control method for Smilax glabra;contents of astilbin and engeletin in Smilax glabra from different producing areas are quite different.

关 键 词：土茯苓 落新妇苷 黄杞苷 高效液相色谱法 不同产地中药材含量差异 

分 类 号：R917[医药卫生—药物分析学]