苦碟子药材及其注射液中化学成分的HPLC-ESI-MS^n分析  

Identification of chemical component from Ixeris sonchifolia and its injection by HPLC-ESI-MS^n

作　　者：刘颖[1,2] 卢建秋[1] 张加余[1,2] 王放[2] 王青[2] 乔延江[2] 

机构地区：[1]北京中医药大学科研实验中心,北京100029 [2]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2013年第16期2675-2681,共7页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2012CB518406)

摘　　要：应用HPLC-ESI-MSn建立1种苦碟子药材及其注射液的快速定性分析方法。通过与对照品保留时间、多级质谱信息对照,快速定性分析苦碟子药材及其注射液中的化学成分。共鉴定出33个化合物,核苷类3个、有机酸类16个、黄酮类7个以及倍半萜内酯类7个,其中通过对照品比对准确鉴定了12个化合物,11个化合物为首次在苦碟子中发现。结合已知化合物的裂解规律和未知化合物的多级质谱信息,对5个同分异构体的化合物结构进行了预测。所建立的苦碟子HPLC-ESI-MSn定性分析方法简单、快速、高效,可以为苦碟子的质量控制和评价提供有效依据。A rapid analytical method was developed for the identification of components in Ixeris sonchifolia and its injection by HPLC-ESI-MSn. The characterization of various components was analyzed according to the retention time of reference standard and mass spectrometry information. A profile of I. sonchifolia and its injection constituents was described and a total of three nucleosides, sixteen phenolic acids, seven flavonoids and twelve sesquiterpene lactones were identified or tentatively characterised. Twelve com- pounds were accurately identified with reference standards. Eleven of them, guanosine, 1-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, caffeic anhydride, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3-p-coumaroylquinic acid, 3-feru- loylquinic acid, 5-p-coumaroylquinic acid and 4-feruloylquinic acid were reported for the first time in I. sonchifolia and its prepara- tions. The structures of five isomers were deduced by theirs mass information and the fragmentation pattern of known compounds. The developed method was useful for the quality control and evaluation of this herb and its preparations. The HPLC-ESI-MSn could be a promising tool for the rapid analysis of components from herbal medicines.

关 键 词：苦碟子 HPLC-ESI-MSn 裂解规律 核苷 有机酸 黄酮 倍半萜内酯 

分 类 号：R284[医药卫生—中药学]