基因芯片技术筛选人参皂苷Rg_1促进人神经干细胞增殖的分子靶点研究  

Study on molecular target promoting human neural stem cells of ginsenoside Rg_1 by gene chip

作　　者：李英博[1] 赵香琴[1] 姜英虹[1] 陈笛[1] 王莎莉[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆市神经生物重点实验室,重庆400016

出　　处：《中国中药杂志》2013年第16期2701-2705,共5页China Journal of Chinese Materia Medica

基　　金：重庆市自然科学基金项目(cstc2011jjA10031)

摘　　要：目的:采用基因芯片技术筛选出人参皂苷Rg1促进人神经干细胞(neural stem cells,NSCs)增殖的主要分子靶点。方法:首先通过MTT法筛选出Rg1促进NSCs增殖的最佳作用质量浓度为120 mg·L-1。然后通过基因芯片技术,观察Rg1促其增殖7 d时靶基因表达,通过数据演算筛选出Rg1促进NSCs增殖的最主要的靶基因和信号转导途径。结果:在Rg1促进NSCs增殖第7天时,获得差异基因440个,其中显著上调的基因266个,显著下调的基因174个;HES1基因和CAMP(环磷酸腺苷)-PKA(蛋白激酶A),PI3K(磷脂酰肌醇-3激酶)-AKT信号传导通路与Rg1促进人NSCs增殖密切相关。结论:基因芯片筛选出的差异表达基因可能为研究Rg1促进NSCs增殖的分子机制研究提供线索。Objective： To screen out main molecular target promoting human neural stem cells （NSCs） of ginsenoside Rgl by using the gene chip technology. Method： First, MTr assay was adopted to screen out the optimal concentration of Rgl-promoted NSC proliferation （120 mg·L-1 ）. Then, on the 7th day after the Rgl-promoted NSC proliferation, the expression of target genes was ob- served by the gene chip technology. The most important target gene and signal transduction pathways were screened out through the data calculations. Result： On the 7^th day after the Rg1-promoted NSC proliferation, obtained 440 differential genes, 266 significantly up- regulated genes and 174 significantly down-regulated genes. HES1 gene, CAMP （cyclic adenosine monophosphate）-PKA （protein ki- nase A） and PI3 K （phosphatidylinositol 3 kinase）-AKT signal transduction pathways were closely related to the NSC proliferation. Conclusion： The differentially expressed genes screened out by gene chip may provide new clues for studies on molecular mechanism of ginsenoside Rgl-promoted NSCs proliferation.

关 键 词：人参皂苷RG1 人神经干细胞 增殖 基因芯片 

分 类 号：R285.5[医药卫生—中药学]