小半夏加茯苓汤中鸟苷含量的高效液相色谱测定  

Determination of guanosine in Xiaobanxia - Fuling Decoction by HPLC

作　　者：祝军委[1] 冯果[1] 冯泳[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《时珍国医国药》2013年第8期1818-1820,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30960472/C190105);贵州省中药现代化专项研究课题(黔科合社字[2008]5025号)

摘　　要：目的建立小半夏加茯苓汤的乌苷含量测定方法。方法采用HPLC测定小半夏加茯苓汤中鸟苷的含量。色谱柱C18(250 mm×4.6 mm,5μm);流动相为乙腈∶水=(4∶96),流速0.7 ml/min,检测波长为260 nm,进样量为10μl,柱温为30℃。结果鸟苷含量在0.49～4.9μg范围内与峰面积呈良好线性关系,回归方程为Y=353 311X+27 1137(r=0.999 9,n=5),平均回收率为99.57%,RSD为1.01%。结论该法简便可行,结果可靠,重复性好,可作为研究小半夏加茯苓汤中鸟苷含量的方法。Objective To establish an HPLC method for determination of guanosine in Xiaobanxia - Fuling Decoction. Methods - Determination of the content of guanosine in Xiaobanxia - Fuling Decoction Column Cls （250 mm ×4.6mm,5μm） was used with acetonitrile - water（4： 96） as mobile phase . The flow rate was 0.7 ml/min,the wavelength was 260 nm, the injection volume was 10μl, and the column temperature was at 30℃. Results The linear range of guanosine was within 0. 49 - 4.9μg, r = 0. 999 9, the average recovery was 96.18% （n = 5 ）. Conclusion This method has good reproducibility,which can be used for quality controlin production of Xiaobanxia - Fuling Decoction.

关 键 词：高效液相色谱 小半夏加茯苓汤 鸟苷 

分 类 号：R284.2[医药卫生—中药学]