银杏叶总黄酮闪式提取工艺的正交设计法优化  

Optimum homogenate extracting process for flavonoids from Ginkgo leaf by orthogonal design

作　　者：赵宏[1] 张宇[1] 王宇亮[1] 唐雨薇[1] 唐丽薇[2] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]昆明理工大学,云南昆明650093

出　　处：《时珍国医国药》2013年第8期1852-1853,共2页Lishizhen Medicine and Materia Medica Research

基　　金：黑龙江自然科学基金面上项目(No.D201064);黑龙江省卫生厅科研项目(No.2011-449)

摘　　要：目的优选银杏叶总黄酮的最佳闪式提取工艺。方法采用闪式提取技术,以黄酮提取率为指标,以乙醇体积分数、提取时间、料液比为主要考察因素,采用L9(33)正交实验法优选银杏叶总黄酮的闪式提取工艺。结果当乙醇体积分数为60%,闪式提取时间30 s,料液比1∶20时,银杏叶总黄酮的提取率最高,为1.99%。结论闪式提取法适合于银杏叶总黄酮的提取,该工艺具有稳定、快速、便捷的优点。Objective To optimize homogenate extracting process of total flavonoids from Ginkgo leaf. Methods By taking the extration of the flavonoids as evaluation parameter, considering the concentration of alcohol, solid to liquid ratio and extraction time as affecting factors, the homogenate extracting process of total flavonoids from Ginkgo leaf is optimized by L9 （33） orthogonal design. Results The optimum condition is achieved when the lever of ethanol is 60% , extraction time is 30 s, and solid to liquid ratio （g/ ml） is 1： 20, the extraction content of total flavonoids is 1.99%. Conclusion The homogenate extraction is feasible and reliable for extracting total ilavonoids from Ginkgo leaf. It is a rapid and high efficient method.

关 键 词：银杏叶 总黄酮 闪式提取法 提取工艺 

分 类 号：R282.5[医药卫生—中药学]