丹葛解酲汤对酒精性肝损害大鼠肝细胞中Bcl-2和NF-kB表达的影响  

The Dang Jiecheng Decoction to big rat liver alcoholic liver cell Bcl - 2 and NF - κB expression's influence

作　　者：马丽[1] 安超[2] 高玉杰[1] 钱月慧[1] 牛阳[1] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏人民医院,宁夏银川750021

出　　处：《时珍国医国药》2013年第8期1854-1856,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家中医药管理局专项课题(No.06-07JP47);宁夏自然科学基金资助课题(No.C03050301)

摘　　要：目的通过观察肝组织病理形态学改变,来探讨丹葛解酲汤对酒精性肝损害大鼠肝细胞中Bcl-2和NF-κB表达的影响。方法将90只SD大鼠随机分为正常对照组、模型组、丹葛解酲汤组,采用白酒灌胃的方法建立大鼠酒精性肝损伤模型,12周后,采用TUNEL法检测肝细胞凋亡指数并观察肝脏的病理学形态;用免疫组化法测定Bcl-2和NF-κB在肝细胞中的表达。结果丹葛解酲汤组就细胞凋亡指数低于模型组,差别有统计学意义(P<0.01);肝组织病理结果提示丹葛解酲汤对酒精性肝损伤大鼠具有保护作用。模型组Bcl-2和NF-kB表达强度均明显高于正常对照组(P<0.01),丹葛解酲汤组Bcl-2表达强度高于模型组(P<0.05),丹葛解酲汤组NF-κB表达强度低于模型组(P<0.01)。结论丹葛解酲汤对酒精性肝损伤大鼠肝细胞具有保护作用;通过影响大鼠肝组织中Bcl-2和NF-κB表达,推测丹葛解酲汤有减少肝细胞凋亡而起到保护肝细胞作用的可能机制。Objective To observe the pathological changes of liver tissue to explore the Dang Jiecheng Decoction to big rat liver alcoholic liver cell Bcl -2 and NF - kB expression＇s influence. Methods 90 SD rats were randomly divided into normal control group, model group, the Dang Jiecheng Decoction group, the method used to establish liquor fed alcohol - induced liver injury model in rats, 12 weeks after detection of liver cells using TUNEL apoptotic index and to observe the liver pathology patterns;Using immunohistochemical determination of Bcl - 2 and NF - κB in liver cells. Results The Dang Jiecheng Decoction group on the apoptotic index lower than the model group, the difference was statistically significant （ P 〈 0.01 ） ; Liver pathology results prompted of Dange Jiechen Decoction has a protective effect of alcohol - induced liver injury in rats. Model group, Bcl - 2 and NF - κB expression intensity were significantly higher than the normal control group （P 〈 0.01 ） higher than that in the model group （P 〈 0.05 ）, the Dan Ge Jie Cheng soup group of Bcl - 2 expression intensity the Dang Jiecheng Decoction group NF - κB expression intensity lower than the model group （P 〈 0.01 ）. Conclusion By the Dang Jiecheng Decoction of alcoholic liver injury in rat liver cells have a protective effect ; Rat liver tissue by affecting the expression of Bcl - 2 and NF - κB, speculated the the Dang Jiecheng Decoction reduce liver cell apoptosis in the sky to protect the liver ceils and possible mechanisms.

关 键 词：丹葛解酲汤 酒精性肝损害 BCL-2 NF—κB 

分 类 号：R285.5[医药卫生—中药学]