Cry1Ah蛋白通过三级食物链对龟纹瓢虫幼虫的影响  

Tritrophic Effects of the Bt-Cry1Ah Protein on the Predator Propylea japonica

作　　者：路献勇[1] 张帅[1] 吕丽敏[1] 雒珺瑜[1] 王春义[1] 崔金杰[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2013年第4期283-290,共8页Cotton Science

基　　金：农业部转基因生物新品种培育重大专项(2013ZX08011-002)

摘　　要：使用生物测定方法评价了Cry1Ah蛋白和食料对龟纹瓢虫幼虫的影响。研究结果表明,用1.0和10.0μg.g-1的Cry1Ah蛋白处理下的棉铃虫饲喂龟纹瓢虫后,其存活率、幼虫虫体质量、幼虫发育时间和化蛹率均没有影响,但龟纹瓢虫体内某些酶活力有所改变,这种改变并未显著影响幼虫的生长发育。用蚜虫饲喂的龟纹瓢虫存活率、虫体质量和化蛹率显著高于棉铃虫处理,差异达显著水平;幼虫发育时间前者显著短于后者。研究结果表明食料的不同对龟纹瓢虫的影响远远大于Cry1Ah蛋白对其的影响。We conducted a tritrophic bioassay to evaluate the potential impact of the CrylAh protein on growth and the activities of development enzymes in Propylea japonica using cotton bollworm （Helicoverpa armigera） as prey. There were no significant differences in the survival rate, larval weight, larval development time, and pupation rate among P. japonica fed with cotton boll- worm larvae reared an artificial diet containing Cryl Ah protein at concentrations of 0 （control）, 1.0, and 10.0 μg. g-1. The activi- ties of some enzymes differed among the P. japonica fed with the different diets, but this did not affect the growth and develop- ment of the larvae. P. japonica fed with aphids showed significantly higher survival rate, larval weight, and pupation rate, and significantly shorter larval development time, compared with those of P. japonica fed with cotton bollworm. These results showed that different diets had a far greater effect on P. japonica than did the different concentrations of the CrylAh protein.

关 键 词：Cry1Ah蛋白 棉铃虫 龟纹瓢虫 生物测定 三级营养 

分 类 号：S562[农业科学—作物学]