雷蒙德氏棉3条染色体特异BAC的筛选与定位  

Screening and Positioning of Three Chromosome-specific BAC Clones in Gossypium raimondii

作　　者：覃琴[1] 刘方[1] 甘仪梅[2] 王春英[1] 王玉红[1] 蔡小彦[1] 王坤波[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物遗传资源重点实验室,海南海口571101

出　　处：《棉花学报》2013年第4期323-328,共6页Cotton Science

基　　金：国家863计划(2012AA101108-02-05);农业部保种项目(NB2012-2130135-29B);农业部948项目(2011-G1-04)

摘　　要：通过筛选海岛棉BAC文库并结合BAC-FISH技术成功获得雷蒙德氏棉D501、D502和D510染色体的特异BAC克隆,可作为这3条染色体的细胞学标记。这3个克隆在雷蒙德氏棉和海岛棉中进行比较作图,结果表明,它们在海岛棉中均表现出了染色体特异性,说明这3个BAC也可用于海岛棉Db01、Db02和Db10染色体的识别。其中BAC克隆280G06在两棉种10号染色体上的位置为非线性关系,可能在棉属的进化过程中发生了染色体结构的变异。We screened a bacterial artificial chromosome（BAC） library of Gossypium barbadense acc. Pirna 90-53 to identify chromosome-specific BAC clones. Using BAC-fluorescence in situ hybridization technology, we obtained three BAC clones specific to chromosome Ds01, D502, and DsI0 of Gossypium raimondii, which could be used as cytological markers for those three chromosomes. Comparative mapping of these three BACs between G. barbadense and in G. raimondii showed that these three BAC clones could also be used to identify chromosomes Db01, Db02, and Dbl0 in G. barbadense. The position of the BAC clone 280G06 on chromosome 10 did not show colinearity between G. barbadense and G. raimondii, possibly because of chro- mosomal rearrangement.

关 键 词：雷蒙德氏棉 单染色体 细菌人工染色体 荧光原位杂交 

分 类 号：S562[农业科学—作物学]