超高效液相色谱法测定白芍单煎液中4种活性成分及与甘草配伍前后含量的变化  

Determination of four major constituents in single herb Paeony decoction and the change before and after compatibility of Radix Paeoniae Alba with Radix Glycyrrhizae by UPLC-PDA

作　　者：李军[1] 甘平平[1] 钟美佐[1] 黄熙[2] 孙明[3] 王杨[2] 夏子安[2] 

机构地区：[1]中南大学湘雅医院肿瘤科,湖南长沙410008 [2]中南大学湘雅医院中西医结合研究所,湖南长沙410008 [3]中南大学湘雅医院心血管内科,湖南长沙410008

出　　处：《中国医院药学杂志》2013年第16期1301-1304,共4页Chinese Journal of Hospital Pharmacy

基　　金：中央高校基本科研业务费专项资金资助(编号:2012QNZT110);国家自然科学基金青年基金项目(编号:81202781)

摘　　要：目的:建立UPLC方法测定白芍单煎液中没食子酸、芍药内酯苷、芍药苷及苯甲酸的含量,并探讨白芍与甘草配伍前后含量的变化。方法:采用Waters Acquity UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱;以乙腈-0.5%醋酸(2∶98)为流动相;行梯度洗脱;流速0.3 mL.min-1,柱温40℃,进样量3μL,测定并比较白芍单煎液及芍药甘草汤中4种成分的含量。结果:UPLC方法精密度、回收率、稳定性良好,结果准确可靠。白芍与甘草配伍后合煎液的没食子酸含量升高,而芍药内酯苷、芍药苷和苯甲酸的含量降低,结果有显著性差异(P<0.01)。结论:白芍与甘草配伍合煎后其活性成分的含量发生了变化。OBJECTIVE To develope an ultra performance liquid chromatography detection method for determination of gal- lic acid, albiforin, paeoniflorin and benzoic acid in single herb Paeony decoction and to observe the changes of the content before and after compatibility of Radix Paeoniae Alba with Radix Glycyrrhizae. METHODS The chromatographic separation was performed on an Acquity BEH C18 column （100 mm× 2. 1 mm, 1.7μm）. The mobile phase was a mixture of acetonitrile and 0. 5% aqueous acetic acid with gradient elution （2：98, V/V） at the flow rate of 0. 3 mL.min-1. The column temperature was maintained at 40 ℃, and injection volume was 3μL. The method was employed to analyze the four compounds in single herb Paeony decoction and the changes of its content. RESULTS The ultra performance liquid chromatography detection method was established with good precision, stability and recovery. The results were accurate and reliable. The amount of gallic acid increased while albiflorin, paeoniflorin and benzoic acid decreased after Radix Paeoniae Alba combining with Radix Glycyrrhizae in SGT （P〈0. 01）. CONCLUSION The compatibility of Radix Paeoniae Alba with Radix Glycyrrhizae could result in the changes of the amount of active components of Radix Paeoniae Alba.

关 键 词：白芍 配伍 甘草 芍药甘草汤 超高效液相色谱 

分 类 号：R927.2[医药卫生—药学]