原代神经元转染方法  

Transfection techniques for primary cultured neurons

作　　者：刘俊燕[1,2] 赵凤艳[1,2] 屈艺[1,2] 母得志[1,2] 

机构地区：[1]四川大学华西第二医院儿科,成都610041 [2]妇儿疾病与出生缺陷教育部重点实验室,成都610041

出　　处：《生命的化学》2013年第4期455-460,共6页Chemistry of Life

基　　金：国家重点基础研究发展计划("973"计划)(2013CB967404);国家自然科学基金项目(81172174;81270724;30973236;31171020);教育部长江学者和创新团队基金(IRT0935);高等学校博士学科点专项科研基金(20110181130002);四川省科技厅科技支撑项目(2010SZ0280)

摘　　要：体外培养的原代神经元形态和功能与在体神经元相似,在神经系统疾病研究中能提供准确的生物学信息,是研究神经系统疾病的重要工具。转染技术的发展,为研究原代培养神经元基因和蛋白质功能提供了技术支持。本文总结了近年来原代神经元转染方法的研究进展,比较了不同转染方法如磷酸钙沉淀法、脂质体介导转染、电穿孔法、病毒介导转染等的优缺点,为选择最佳的神经元基因转染技术提供有价值的参考。The primary cultured neurons in vitro have similar morphology and function as those of neurons in vivo. Therefore, it can provide accurate biological informations and is regarded to be an important tool in the studies of nervous system disorders. The development of transfection technology has provided technical supports for functional research of genes and proteins in neurons. Here we will summarize the research progress in transfection methods of primary cultured neurons in recent years, and will compare the advantage and disadvantage among different transfection methods such as calcium phosphate method, lipofection, electroporation and viral transduction. It may be helpful to provide guidance to select the most effective transfection methods of primary cultured neurons.

关 键 词：原代神经元 转染方法 磷酸钙沉淀法 脂质体法 电穿孔法 病毒转染 

分 类 号：Q78[生物学—分子生物学]