橡胶树HbWRKY3的克隆及其响应逆境胁迫的表达分析  

Cloning and Expression Analysis of HbWRKY3 in Hevea brasiliensis Under Abiotic Stress

作　　者：谢黎黎[1,2] 安泽伟[2] 李雅超[1,2] 翟琪麟[1,2] 程汉[2] 方家林[2] 黄华孙[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]国家橡胶树育种中心,中国热带农业科学院橡胶研究所,海南儋州571737

出　　处：《热带作物学报》2013年第8期1463-1472,共10页Chinese Journal of Tropical Crops

基　　金：国家天然橡胶产业技术体系(No.CARS-34);国家自然科学基金项目(No.30960319;31271796);中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022011002)

摘　　要：通过RACE技术扩增获得橡胶树HbWRKY3基因,并对该基因及其所编码氨基酸序列进行生物信息学分析和响应逆境胁迫的表达分析,结果表明:该基因含有2个WRKYGQK结构域和2个C2H2锌指结构,属于WRKY基因家族第Ⅰ类;由4个外显子和3个内含子组成,DNA序列全长3 780 bp;开放阅读框长度为2 211 bp,编码由737个氨基酸组成的多肽。该基因定位于细胞核中,在橡胶树叶片、树皮、花、胶乳等不同组织中都有表达,以花中的表达水平最高。盐、PEG、茉莉酸、水杨酸等非生物胁迫均可诱导HbWRKY3的表达,但干旱、ABA、乙烯和低温胁迫的诱导较为明显。WRKY transcription factor is one of plant-specific transcription factors, which plays important roles in regulating plant growth, development, and response to biotic and abiotic stresses. In this study, HbWRKY3, with full length 3 780 bp DNA of four exons and three introns, was cloned from rubber tree by RACE technology. HbWRKY3 contained a 2 211 bp open reading frame encoding a putative protein of 737 amino acids. HbWRKY3 protein contained two highly conserved WRKYGQK domains and two C2H2 zinc finger domains, and belonged to group I of WRKY superfamily. HbWRKY3 was localized in nucleus. It could be constitutively expressed in leaves, bark, flowers and latex, and the highest expression level was in flowers. The expression analysis of HbWRKY3 showed that HbWRKY3 could be induced by various stresses such as NaC1, PEG, MeJA, and SA, but it could be strongly induced by Eth, drought, low temperature and ABA.

关 键 词：橡胶树 转录因子 HbWRKY3 非生物胁迫 表达分析 

分 类 号：S794.1[农业科学—林木遗传育种]