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机构地区:[1]中国药科大学新药筛选中心,江苏南京210009
出 处:《亚太传统医药》2013年第8期30-33,共4页Asia-Pacific Traditional Medicine
基 金:企业创新药物孵化基地建设(2001ZX09401)
摘 要:目的:研究β-榄香烯体外抗凝血与溶血栓活性以及体内抗凝血,抗血小板聚集作用,为探讨β-榄香烯活血化瘀药效提供参考。方法:采用新西兰兔体外抗凝血法、血浆复钙时间实验及体外血栓法、全血块法研究β-榄香烯的体外抗凝血、溶血栓作用;采用皮下注射盐酸肾上腺素和冰水刺激复制大鼠急性寒凝血瘀证模型,通过观察凝血酶原时间(PT)、凝血酶时间(TT)以及二磷酸腺苷(Adenosine diphosphate,ADP)和花生四烯酸(Arachidonic acid,AA)诱导血小板最大聚集率,研究β-榄香烯口服制剂对血瘀大鼠体内凝血功能的影响。结果:β-榄香烯各剂量组均能延长新西兰兔血浆复钙时间(P<0.05),加快体外血栓及全血凝块的溶解(P<0.05);β-榄香烯口服制剂高剂量组延长寒凝血瘀大鼠的凝血酶原时间(PT)(P<0.05),β-榄香烯口服制剂各剂量组延长寒凝血瘀大鼠的凝血酶时间(TT)(P<0.01),且对ADP和AA诱导的血小板最大聚集率有显著的抑制作用(P<0.01)。结论:β-榄香烯具有抗凝血和溶血栓的作用。Objective:β-elemene has been known to have potential use in antitumor, but its anticoagulant and antithrombotic role has not been well studied. So we investigated the effects of β-elemene on anticoagulant and antithrombotic. Methods : The anticoag- ulant effect of β-elemene in vitro was determined by means of anticoagulant experiment and plasma recalcificatic time. The antithrom- botic effect of was shudied by means of thrombus in vitro and whole blood gore. The acute blood-stasis model rats were made by soaked ice water after injection of adrenalin hydrochloride in a major dose. Using the model acute blood stasis, detecte prothrombin time (PT),thrombin time (TT) and adenosine diphosphate (ADP) and arachidonic acid (AA) were used to induce platelet aggregation to investigate the effects of β-elemene on anticoagulant in vivo. Results:Compared with control group, all the dosages of β-el- emene could not only prolong the plasma recalcificantic time (P〈0.05) but also dissolve the thrombus (P〈0.05) and whole blood gore (P(0.05) in vitro; prolong prothrombin time (P〈0.05) and thrombin time (P〈0.01)and inhibited platelet aggregation with different revulsant and reduced the maximum aggregation rate (P〈0.01) in vivo. Conclusion :β-elemene could obviously inhibit coagulant and hrombotic activities.
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