过氧化物酶体增殖物激活受体-γ及核因子-κB在特应性皮炎患者皮损中的表达及意义  

Expression and role of peroxisome proliferator-activated receptor-γ and nuclear factor-κB in the skin lesions of patients with atopic dermatitis

作　　者：黎志刚[1] 吴江[1] 刘衍彬[1] 许志萍[1] 郑学毅[1] 孙广政[1] 

机构地区：[1]广州市第一人民医院,510180

出　　处：《实用皮肤病学杂志》2013年第3期138-139,142,共3页Journal of Practical Dermatology

基　　金：广东省科技计划项目(2012B031800342)

摘　　要：目的观察过氧化物酶体增殖物激活受体-γ(PPAR-γ)及核因子-κB(NF-κB)在特应性皮炎患者皮损中的表达情况,探讨其在特应性皮炎发病中的作用。方法采用免疫组化Westernblotting法检测22例特应性皮炎患者皮损及非皮损,以及12名正常皮肤组织中PPAR-γ、NF-κB的表达。结果特应性皮炎患者皮损组PPAR-γ的阳性表达计数为0.738±0.069,低于正常对照组的0.869±0.048和非皮损组的0.802±0.026(P<0.05);特应性皮炎患者皮损组NF-κB的阳性表达计数为0.937±0.039,高于正常对照组的0.735±0.015和非皮损组的0.796±0.068(P<0.05);特应性皮炎患者皮损组PPAR-γ和NF-κB表达呈负相关(P<0.05)。结论特应性皮炎患者皮损组PPAR-γ表达减弱、NF-κB表达增强在发病过程中可能起一定的作用,为特应性皮炎患者的临床治疗提供了新的思路。Objective To evaluate the expression of peroxisome proliferator-activated receptor-γ（PPAR-γ） and nuclear factor-κB （NF-κB） in the skin lesions of patients with atopic dermatitis, and to explore their effect on the pathogenesis of atopic dermatitis. Methods Immunohistochemistry andWestern blotting was perform on the investigate the PPAR-γ and NF-κB expression in lesions specimens and non-lesions specimens from 22 cases of atopic dermatitis and 12 cases of normal skin tissue. Results The positive score of PPAR-γ （0.738±0.069） in the skin lesions of patients with atopic dermatitis was lower than the normal control group （0.869±0.048, P〈0.05） and non-lesional group （0.802±0.026, P 0.05）. The positive score of NF-κB （0.937±0.039） in the skin lesions of patients with atopic dermatitis was higher than the normal control group （0.735±0.015, P〈0.05） and non-lesional group（0.796±0.068, P〈0.05）. There was negative correlation between PPAR-γ and NF-κB expression in atopic dermatitis patients with lesions group （P〈0.05）. Conclusion The decreased expression of PPAR-γ, and enhanced expression of NF-κB play a role in the pathogenesis of atopic dermatitis, which may provide a new idea for treating atopic dermatitis.

关 键 词：皮炎 特应性 过氧化物酶体增殖物激活受体-Γ 核因子-ΚB 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]