大鼠急性肺缺血再灌注损伤模型的建立与评估  

Establishment and Evaluation of Acute Lung Ischemia-reperfusion Injury in Rat Model

作　　者：杨世疆[1,2] 王家宁[1] 平伟[1] 江文洋[1] 徐澄澄[1] 陈文树[1] 付向宁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸外科,武汉430030 [2]石河子大学医学院第一附属医院心胸外科,石河子832008

出　　处：《中国医药导刊》2013年第5期858-859,862,共3页Chinese Journal of Medicinal Guide

基　　金：国家自然科学基金资助项目(编号:81000031);石河子大学高层次人才项目(编号:RCZX200687)

摘　　要：目的:探索一种简便制备大鼠急性肺缺血再灌注损伤模型的方法,并对其效果进行评估。方法:将30只Wistar大鼠随机分成2组(缺血再灌注组和假手术组),每组15只。假手术组单纯开胸,缺血再灌注组采用显微无创血管夹夹闭左肺门60min,松开血管夹后再灌注120min,建立急性肺缺血再灌注模型。术后获取标本,检测肺湿干重比值,肉眼、光镜观察肺组织结构变化。结果:肺缺血再灌注组湿干重比值明显高于假手术组(P<0.01),肉眼、光镜下均可观察到肺损伤改变。结论:成功构建了Wistar大鼠急性肺缺血再灌注损伤动物模型,操作简单,成功率较高,为肺缺血再灌注损伤机制研究提供了一种可行的动物模型。Objective：To prepare and evaluate a new type of acute lung I/R injury model in rats.Methods：Thirty Wistar rats were randomly divided into two groups： IR group and sham group.We establish a lung I/R injury model,the rats underwent left lung ischemia,after 60 minutes of ischemia,reperfusion for up to 2 hours was evaluated.The ratio of lung W/D weight was compared between two groups,and the changes in lung structures were observed under the visual inspection and light microscopy.Results：The rate of lung W/D weight in I/R group was significantly higher（P0.01）than in control group.Compared with IR group and sham group in light microscopy,there were more serious damages in IR group.Conclusion：It is simple and easy to establish a model of lung I/R injury in rats,maybe useful in studying the mechanism of lung I/R injury.

关 键 词：肺 缺血再灌注损伤 动物模型 大鼠 

分 类 号：R655[医药卫生—外科学]