β-榄香烯吗素与阿霉素联合应用对CEM/ADM细胞生长的影响  

Effect of β-elemene derivatve in combination with ADM on CEM/ADM cells growth

作　　者：郝立宏[1] 卢步峰[1] 于丽敏[1] 杨佩满[1] 

机构地区：[1]大连医科大学组胚学教研室,辽宁大连116027

出　　处：《大连医科大学学报》2000年第3期165-167,共3页Journal of Dalian Medical University

摘　　要：应用台盼蓝排染法 ,Wirght -Giemsa混合染色法 ,Hoechst3334 2荧光染色法及碘化丙啶(Propidiumiodide ,PI)与Hoechst 3334 2共染计数坏死细胞的PI阳性率测定法 ,研究 β -榄香烯吗素 (PIC -BE)及其与阿霉素 (ADM)联合应用对CEM/ADM细胞生长的影响。结果显示 :①PIC-BE能够显著抑制CEM/ADM细胞的生长 ,并诱导该细胞凋亡 ,其作用强度在一定范围内呈浓度和时间依赖性。②低毒剂量的PIC -BE与ADM联合应用可显著增强ADM对CEM/ADM细胞的生长抑制和凋亡诱导作用 ,其作用强度在一定范围内亦呈浓度和时间的依赖性。提示 :PIC -BE主要以诱导肿瘤细胞凋亡而表现其抗肿瘤作用 。On basis of the past stuby on β-elemene emulsion,we studied the effect of the wate-soluble β-elemene derivative(PIC-BE)from β-elemene emulsion on inhibiting directly and reversal of CEM/ADM MDR cells line by usinging trypan blue,Hoechst 33342 fluorescence and PI stainings.Results:①PIC-BE could inhibit markedly CEM/ADM cells growth,and it's inhibition ratio as while as 60 hr were 27 37,36 84,81 05%respectively segmficanfly higher then that of control( P <0 01),and its apoptosis ratio is 12 9,13 8,23 4,40 2%( P <0 01)in 36 hr with dose and time dependent charecterestics in a linited range.This result suggest that PIC-BE is a kind of antitumor chemical compound.②The effect of low-cytotoxic dose of PIC-BE in combination with ADM on inhibition of cell growth and induction of apoptosis were far more potent than the effect of single ADM( P <0 01),the sum of single ADM and PIC-BE( P <0 01).The effect of PIC-BE 10 0 μg/ml,ADM 1 0 μg/ml,PIC-BE 10 0 μg/ml+ADM 1 0 μg/ml,ADM 2 0 μg/ml,PIC-BE10 0 μg/ml+ADM 2 0 μg/ml in 48 hr on the inhibit ratio of CEM/ADM cell growth is 20 8,37 5,78 3,39 2,88 3%( P <0 01) respectively,the cell apoptosis ratio is 111 6,18 2,41 2,14 2,53 1%( P <0 01),and the growth-inhibitory effect intension of PIC-BE was dose and time-dependent within a limited range.Conclusion:PIC-BE could partially reverse the MDR of CEM/ADM cell,and it has synergistic effect of AMD on anti-tumor.

关 键 词：β-榄香烯吗素 阿霉素 CEM/ADM细胞 生长抑制 

分 类 号：R979.1[医药卫生—药品]