多形拟杆菌对糖尿病模型小鼠的影响  

The effect of Bacteroides thetaiotaomicron on diabetic mouse models

作　　者：谢婧雯[1] 王烨[1] 朱明[2] 刘静[1] 朱曼丽[1] 姬凤彩[3] 毛新民[4] 李琳琳[1] 

机构地区：[1]新疆医科大学基础医学院药理教研室,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院微生物与免疫教研室,新疆乌鲁木齐830011 [3]新疆医科大学第一附属医院动物中心,新疆乌鲁木齐830011 [4]新疆医科大学中医学院,新疆乌鲁木齐830011

出　　处：《中国微生态学杂志》2013年第8期869-873,881,共6页Chinese Journal of Microecology

基　　金：国家自然科学基金(30760288):新疆维吾尔族;哈萨克族2型糖尿病与肠道菌群的相关性研究

摘　　要：目的研究多形拟杆菌(BT)干预糖尿病模型小鼠后对血糖、体重和C肽的影响。方法 (1)用四氧嘧啶(200 mg/kg腹腔注射)制备糖尿病模型小鼠,并分成四组:空白组(n=10)、空白给菌组(n=10)、四氧嘧啶糖尿病模型组(n=11)和模型给菌组(n=11),15 d。(2)BHI血琼脂培养基培养ATCC 29148标准菌株,比浊法测定混悬菌液数量。(3)用多形拟杆菌菌液干预空白给菌组和模型给菌组小鼠,观察15 d中四组小鼠的体重、空腹血糖水平的变化;实时荧光定量PCR测定小鼠肠道内多形拟杆菌的数量;运用酶联免疫法(ELISA)测定血清中C肽的水平。结果 (1)给予菌悬液后,空白给菌组与空白组相比,BT在第3天就可以定植并维持到第15天。与模型组相比,模型给菌组在第15天时可以定植;(2)线性回归相关性分析显示,肠道内多形拟杆菌与体重呈负性相关(r=-0.70,P<0.05);与空腹血糖(FPG)呈正性相关(r=0.71,P<0.05);与C肽呈负性相关(r=-0.62,P<0.05);与胰岛素抵抗指数(HOMA-IR)呈正性相关(r=0.55,P<0.05);与胰岛素分泌指数(HOMA-IS)呈负性相关(r=-0.43,P<0.05)。结论 (1)外源性灌胃给予多形拟杆菌可以在肠道内定植。(2)肠道内多形拟杆菌的数量变化与糖尿病有相关性。Objectives To study the effect of Bacteroides thetaiotaomicron （BT） intervention on the blood glucose, body weight and C-peptide level in diabetic mice. Methods （ 1 ） Diabetic mouse models were injected alloxan for 15 days （200 mg/kg intraperitone- al injection） and divided into four groups： control group （n = 10） , bacteria-injected control group （n = 10）, alloxan-induced diabetic model group （n = 11 ） and bacteria-injected model group （n = 11 ）. （2） ATCC 29148 standard strains were cultured on BHI solid medi- um; bacteria count was determined using nephelometry method. （3） The bacteria-injected control and model groups were injected Bacte- roides thetaiotaomicron suspension, and the changes of body weight, fasting blood glucose （FBG） levels of four groups were detected. The amount of bacteroides was determined by Real-time PCR; enzyme-linked immunosorbent assay （ELISA） was used to detect the serum C- peptide levels. Results （ 1 ） Comparing with the control group, BT colonization appeared at Day 3 and still maintained at Day 15 in bac- teria-injected control group. Comparing with the model group, BT colonization appeared at Day 15 in alloxan-induced diabetic model group. （2） Linear regression correlation analysis showed that Bacteroides thetaiotaomicron was negatively correlated with body weight （r = -0.70, P 〈 0.05） while positively correlated with FBG （r = 0. 71, P 〈 0. 05）; C-peptide showed a negative correlation （ r = - 0. 62, P 〈0. 05 ） ; HOMA-IR was positively correlated （ r = 0. 55, P 〈 0.05 ） and HOMA-IS showed a negative correlation （r = -0. 43, P 〈 0. 05）. Conclusion （1）Bacteroides thetaiotaomicron can colonize the intestine via exogenous administration. （2） Bacteroides thetaiotaomicron number changes are associated with diabetes.

关 键 词：四氧嘧啶糖尿病模型 多形拟杆菌 血糖 体重 

分 类 号：R378.2[医药卫生—病原生物学]