复方清下汤对脓毒症大鼠肺组织细胞因子白介素-1及白介素-6基因表达的影响  

Effect of Fufang Qingxia Tang on the expression of IL-1 and IL-6 in sepsis induced acute lung injury

作　　者：孙媛[1] 张丽[1] 李宏[1] 陈晓燕[1] 王晓炜[1] 吕朋[2] 张日欣[2] 刘毅[2] 孙伟[2] 陈海龙[3] 苗健[2] 

机构地区：[1]大连医科大学生物学教研室,辽宁大连116044 [2]大连医科大学第二临床学院普外科,辽宁大连116027 [3]大连医科大学第一临床学院普外科,辽宁大连116011

出　　处：《中国微生态学杂志》2013年第8期887-889,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金(30700365);国家自然科学基金(81173452);辽宁省自然科学基金(201202046)

摘　　要：目的探讨复方清下汤对脓毒症大鼠肺组织白介素-1(IL-1)及白介素-6(IL-6)基因表达的影响,进一步探讨其减轻肺损伤机制。方法将健康SD大鼠随机分为4组,每组10只:假手术组(SHAM组),脓毒症肺损伤组(模型组),盲肠结扎穿孔+复方清下汤组,以及盲肠结扎穿孔+头孢哌酮舒巴坦(舒普深)组,造模24 h后收集标本。应用免疫组织化学和Western blotting法检测肺组织中IL-1、IL-6的表达,RT-PCR检测肺组织上述蛋白mRNA表达。结果与SHAM组比较,模型组IL-1、IL-6的mRNA转录水平和蛋白水平表达均显著升高(P<0.01);抗生素及中药处理组与模型组比较,IL-1、IL-6的表达明显降低(P<0.01),抗生素及中药处理组两组检测数据相近。结论脓毒症大鼠肺损伤时细胞因子IL-1、IL-6过度表达可能是造成脓毒症肺损伤的重要原因;复方清下汤处理的动物模型肺损伤减轻的同时IL-1、IL-6表达变化,提示它可能通过调控IL-1、IL-6表达起作用。Objective To investigate the effect of Fufang Qingxia Tang （FFQX） on the expression of IL-1 and IL-6 in sepsis in- duced acute lung injury （ALI）. Methods SD rats were randomly divided into four groups ： SHAM group （ n = 10 ）, ALI group （ n = 10）, FFQX group （ n = 10）, and Antibiotics （ALI ＋ Cefoperazone-Sulbactam） group （ n = 10 ）. Specimen were collected 24 hours after ALI. IL-1 and IL-6 in lung tissue were measured via immunohistochemistry and Western blotting, respectively. RT-PCR were performed to measure mRNA of these mediators. Results The increased expression of IL-1 and IL-6 in ALI group compared with SHAM group （P 〈 O. 01 ）. The protein and mRNA expression of IL-1 and IL-6 dropped significantly in FFQX group and Antibiotics group com- pared with ALI group （ P 〈 0. 01 each）, although there were no obvious difference between FFQX group and Antibiotics group. Conclusion FFQX can inhibit the expression of IL-1 and IL-6 and thus relieve sepsis induced lung injury.

关 键 词：脓毒症 急性肺损伤 白介素-1 白介素-6 复方清下汤 

分 类 号：R563.2[医药卫生—呼吸系统]