支气管哮喘患者粒-巨噬细胞集落刺激因子mRNA表达的研究  

Expression of GM CSF mRNA in asthmatic patients

作　　者：张伟[1] 钟南山[2] 徐军[2] 

机构地区：[1]广东省人民医院呼吸科,510080 [2]广州呼吸疾病研究所,510120

出　　处：《中华结核和呼吸杂志》2000年第9期534-537,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金："九五"国家医学科技攻关计划项目资助! (96 90 6 0 2 18)

摘　　要：目的　探讨利用无创伤性方法寻找较为特异性地反映哮喘患者气道炎症的客观指标。方法　通过研究哮喘患者气道粘膜细胞粒 巨噬细胞集落刺激因子 (GM CSF)mRNA表达 ,并以此为参照 ,分析哮喘患者诱导痰细胞GM CSFmRNA表达变化 ,利用免疫组化结合逆转录聚合酶链反应(RT PCR)技术 ,分析哮喘组 (10例 )气道粘膜细胞GM CSFmRNA的表达 ;用 3%高渗盐水诱导痰液 ,利用RT PCR技术分析哮喘组 (15例 )、慢性支气管炎组 (15例 )和对照组 (11例 )诱导痰细胞GM CSFmRNA表达。结果　 (1)哮喘组气道粘膜嗜酸细胞、淋巴细胞和活化嗜酸细胞 (EG+2 细胞 )分别为(17 9± 7 0 )× 10 2 、(36± 13)× 10 2 和 (8 9± 3 0 )× 10 2 个 /mm2 ,对照组分别为 (1 9± 1 0 )× 10 2 、(6± 5 )× 10 2 和 (0 8± 0 2 )× 10 2 个 /mm2 ,EG2 细胞数与病情程度呈正相关 (r=0 82 ,P <0 0 5 ) ;(2 )哮喘组气道粘膜细胞GM CSFmRNA表达为 (1 4± 0 6 ) ,与对照组 (0 3± 0 3)比较 ,差异有显著性 (P <0 0 5 ) ,并且GM CSFmRNA的表达与EG+2 细胞数明显相关 (r=0 73 ,P <0 0 5 )。 (3)哮喘组诱导痰嗜酸细胞相对计数为 (0 334± 0 0 6 7) ,与慢性支气管炎组的 (0 0 2 1± 0 0 0 4)和对照组的 (0 0 0 8± 0 0 0 3)比较 。Objective To explore non invasive and specific markers for airway inflammation. Methods RT PCR methods were used to measure GM CSF mRNA expression in airway mucosa and induced sputum from asthmatic patients. The correlation was analyzed between expression of GM CSF mRNA and eosinophils or other inflammatory cells.Results (1) The numbers of eosinophils and EG 2 positive cells were (17.9±7.0)×10 2 per mm 2, and (8.9±3.0)×10 2 per mm 2 in the asthmatic group, significantly higher than those in the non asthmatic control which were (1.9±1.0)×10 2 per mm 2, and (0.8±0.2)×10 2 per mm 2, respectively ( P <0.05). (2) The expression of GM CSF mRNA was (1.4±0.6) in the asthmatic group, significantly higher than that in the control group (0.3±0.3) ( P <0.05), and GM CSF mRNA expression was correlated with the numbers of EG 2 positive cell ( r =0.73 and P <0.05). (3) The percentage of eosinophils in induced sputum from asthmatic patients was (0.334±0.067), which was significantly higher than that from chronic bronchitis and control (0.021±0.004), (0.008±0.003)( P <0.05). (4) The expression of GM CSF mRNA in asthmatics was (0 320±0 054), significantly higher than that in chronic bronchitis and the control group (0.188±0 024 and 0 058±0 028) ( P <0.05). The expression of GM CSF mRNA in patients with chronic bronchitis was also significantly higher than that in the control group ( P <0.05). Conclusions The expression of GM CSF mRNA in induced sputum may be a relatively specific marker for airway inflammation.

关 键 词：支气管哮喘 GM-CSF MRNA 气道炎症 

分 类 号：R562.25[医药卫生—呼吸系统]