金纳多对非协调性异种大鼠肝移植缺血再灌注损伤的防治作用  

Protective effect of Ginaton on rat liver microcirculation disturbance following liver xenotransplantation

作　　者：张群华[1] 芮晓晖[1] 蔡端[1] 孙鹏[1] 张延龄[1] 

机构地区：[1]上海医科大学附属华山医院外科,200040

出　　处：《中华医学杂志》2000年第9期706-708,共3页National Medical Journal of China

基　　金：上海市科委重点基金资助项目! (98XD14 0 0 2 )

摘　　要：目的　探讨金纳多对非协调性异种肝移植缺血再灌注损伤的防治作用。方法　对豚鼠和SD大鼠非协调性异种肝移植进行金纳多 (银杏黄酮配糖体 )注射液治疗的分析 ,实验分为术前、术后金纳多治疗组和对照组 ,观察异种肝移植后肝脏和心脏的病理变化以及肝脏门脉血流变化。结果肝移植术后 ,两组的肝脏和心脏病理变化相似 ,肝内小血管腔内血小板、中性粒细胞聚集和纤维蛋白沉着造成广泛血栓 ,引起血管阻塞和组织梗死。心肌细胞破坏 ,心肌纤维排列紊乱。而术前金纳多治疗组 ,移植肝内小血管血小板和中性粒细胞聚集程度减轻 ,心肌细胞损害也明显好转。多普勒超声检测发现金纳多可以减轻异种肝移植后肝脏的灌注障碍。结论　金纳多对非协调性异种肝移植缺血再灌注损伤有保护作用。并减轻其超急性排斥反应 。Objective To study the effect of Ginaton on liver microcirculation disturbance following liver xenotransplantation in rats. Methods Ginaton was injected intravenously to recipients at a dosage of 14 mg/kg before graftng liver of guinea pigs. The portal blood flow(PBF) was measured by Doppler ultrasound at 0, 30 and 60 minutes after transplantation, and the pathologic changes of liver and heart were observed. Results The reperfusion status of control group was poor and leukocyte infiltration appeared in the center of lobute following transplantation and myocardial cells were impaired. Significant reduction in PBF was found in rats following transplantation. Ginaton pretreatment distinctly ameliorated PBF. The speed of PBF was closely correlated with the pathologic changes following transplantation. Conclusion In the process of liver transplantation, ischemia reperfusion damage may lead to xenoheptic microcirculaton disturbance. Ginaton could improve the xenoheptic microcirculation and reduce ischemic reperfusion damage.

关 键 词：肝移植 再灌注损伤 异种移植 防治 金纳多 

分 类 号：R657.3[医药卫生—外科学]