辛伐他汀对炎症诱导的人脐静脉内皮细胞蛋白C受体mRNA表达的影响  

Effects of TNF-α and Simvastatin on expression of EPCR mRNA in human umbilical vein endothelial cells

作　　者：郭小芳[1,2] 刘海波 任惠龙 赖静波 龚维坤 成兴波[2] 卢国元[4] 

机构地区：[1]浙江宁波市鄞州人民医院内分泌科,宁波315040 [2]苏州大学医学院附属第一医院内分泌科,苏州215000 [3]浙江宁波市鄞州人民医院心内科,宁波315040 [4]苏州大学医学院附属第一医院肾内科,苏州215000

出　　处：《新医学》2013年第8期569-573,共5页Journal of New Medicine

摘　　要：目的探讨辛伐他汀对TNF-α诱导的内皮细胞炎症的抑制作用。方法通过体外培养人脐静脉内皮细胞(ECV304),分别以流式细胞术和逆转录PCR技术证实ECV304细胞膜上内皮细胞蛋白C受体(EPCR)蛋白和mRNA水平的表达。再分别以含不同浓度TNF-α(2、10、25)ng/ml的培养基以及含或不含辛伐他汀(0.1、1、10)μmol/L的TNF-α(10 ng/ml)培养基孵育ECV304细胞12 h,行剂量和时间依赖性实验。采用实时定量PCR技术测定ECV304 EPCR mRNA的表达。结果 EPCR在ECV304上高表达。TNF-α能显著下调ECV304 EPCR mRNA的表达,并呈剂量和时间依赖性(P<0.05)。在采用辛伐他汀干预后,10 ng/ml TNF-α处理的ECV304细胞EPCR mRNA表达的下调得到明显改善。结论 TNF-α对EPCR mRNA表达的下调可能是其损害内皮细胞功能的新机制。辛伐他汀可阻止TNF-α诱导的ECV304上EPCR mRNA表达的下调,从而保护内皮细胞功能。Objectives To investigate the effects of tumor necrosis factor alpha and Simvastatin on the expression of endothelial protein C receptor（EPCR） in hmnan umbilical vein endothelial cells （ECV304）. Methods EPCR expression and mRNA levels was detected with flow cytometry technique and reverse tran- scription-polymerase chain reaction （RT-PCR） technique respectively in ECV304 （：ells. Different concentra- tions of TNF-oLand Simvastatir were added to the culture medium to observe their influence on EPCR expres- sion. The mRNA levels of EPCR were measured by quantitative real-time PCR. Results ECV304 expressed EPCR mRNA at a high level. The expression of EPCR in ECV304 was clown-regulated by TNF-α in a dose and time dependent pattern. Simvastatin could reverse the down-regulative effect of TNF-α on f EPCR mRNA ex- pression in ECV304. Conclusion Down-regulation of EPCR may be a new mechanism of TNF＇s damage to en- dothelial cells. Simvastatin can attenuate the injury of ECV304 induced by TNF-α and might protect the func- tion of endothelial cells.

关 键 词：内皮细胞 肿瘤坏死因子 辛伐他汀 内皮细胞蛋白C受体 

分 类 号：R96[医药卫生—药理学]