利用RSLC建立地骨皮药材的特征指纹图谱研究  

Study on RSLC Fingerprint of Lycii Cortex

作　　者：赵晓玲[1] 姚霞[1] 张鑫瑶[1] 马培[1] 何春年[1] 彭勇[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所/国家教育部中草药物质基础与资源利用重点实验室,北京100193

出　　处：《中药材》2013年第6期895-899,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30530860)

摘　　要：目的:建立地骨皮药材的超快速液相色谱(RSLC)特征指纹图谱,检验地骨皮来源品种的成分区别,控制地骨皮商品药材的质量。方法:采用AcquityUPLCC18色谱柱(2.1mm×100mm,1.7μm),流动相为乙腈-0.1%乙酸水溶液,梯度洗脱,流速0.3mL/min,柱温为40℃,检测波长315nm。利用聚类分析、主成分分析、特征图谱相似度分析3种方法对不同产地的地骨皮药材进行评价。结果:建立了地骨皮药材的RSLC特征指纹图谱,标定了14个共有峰。聚类分析和主成分分析结果表明,地骨皮药材2个来源品种枸杞和宁夏枸杞之间仅在含量方面存在一定差异,所含化合物的种类差别不大。结论:本研究建立地骨皮药材的特征指纹图谱,特征性和专属性强,且方法快速、简便、可靠,有助于深入评价地骨皮药材质量。Objective：To develope a method of RSLC fingerprint analysis for the quality evaluation of Lycii Cortex,and assess the differences of Lycii Cortex from Lycium chinense and L. barbnrum. Methods： All separations were performed using a Rapid Separation Liquid Chromatography （RSLC） system with an Acquity UPLC C18 （2. 1 mm × 100 mm, 1.7 μm） column. The mobile phase was consisted of acetonitrile and 0. 1% aqueous acetic acid. The flow rate was 0. 3 mL/min using gradient elution. The column temperature was 40 ℃ and detection wavelength was set at 315 nm. Lycii Cortex samples collected from different habitats were assessed by similarity analysis （SA）, hierarchical clustering analysis （HCA） and principle component analysis （PCA）. Results：The characteristic RSLC fingerprint was established with 14 common peaks. L4 batehes of samples were classified as 2 clusters by HCA and PCA, and the result demonstrated that there were differences in the contents of chemical composition between 2 species of Lycii Cortex. Cnnclusion：The established RSLC fingerprint is specific and the method is rapid, simple and reliable,which can be used to control the quality of Lycii Cortex in the markets.

关 键 词：地骨皮 超快速液相指纹图谱 聚类分析 主成分分析 质量控制 

分 类 号：R282.5[医药卫生—中药学]