基于主成分分析的茶叶预防肺癌药效物质研究  

Study on Effective Substances of Tea for Chemoprevention of Lung Cancer Based on Principal Component Analysis

作　　者：金苹[1,2] 朱粉霞[1] 谭晓斌[1] 刘文博[1] 金鑫[1] 封亮[1] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院/国家中医药管理局中药释药系统重点实验室,江苏南京210028 [2]江苏大学药学院,江苏镇江212003

出　　处：《中药材》2013年第6期948-952,共5页Journal of Chinese Medicinal Materials

基　　金：国家中医药管理局中药释药系统重点研究室开放课题(2011-2013);江苏省中医药科学技术专项(LZ11062)

摘　　要：目的:采用主成分分析(PCA)法研究6种茶叶预防肺癌的药效物质基础。方法:以UPLC对各茶叶中各成分进行含量测定,MTT法检测CSE诱导的NHBE细胞的存活率,5,5-二硫二硝基苯甲酸(DTNB)比色法测定GSH/GSSG(还原型/氧化型谷胱甘肽)的比例,采用主成分分析计算共有峰面积的相关系数矩阵、特征值和方差贡献率,确定其具有预防肺癌的化学成分。结果:6种茶叶中龙井保护NHBE细胞损伤的作用最强,其IC50为0.2559mg/mL,使GSH/GSSG的比例由0.142恢复至0.858,且具有剂量依赖性;主成分分析得出GCG预防肺癌的贡献率为79.602%,GA的贡献率为11.307%,两者之和为90.909%。结论:不同品种茶叶对NHBE细胞的保护作用不同,主成分分析法确定GCG和GA是茶叶中预防肺癌的主要活性成分。Objective：To study the effective substances of tea for chemoprevention of lung cancer based on Principal Component Analysis （PCA） . Methods ： UPLC was used to determine the content of each component in tea, MTT was used to analyze the survival of CSE-induced NHBE cells. DTNB and the colorimetric assay were used to detect the rate of GSH/GSSG,PCA was used to calculate the correlation coefficient matrix,eigenvalues and variance of contribution ratio to chemoprevention of lung cancer. Results： Longiing （L） was the most effective one to protect NHBE cells from damaging,and the ICs0 of L for prevention of NHBE cells was 0. 2559 mg/mL and could make the GSH/GSSG ratio recovery ranging from 0. 142 to 0. 858 in a dose-dependent manner. The contribution of GCG prevention of lung cancer was 79. 602% and that of GA was 11. 037% by PCA. Conclusion： Different kinds of tea have different protective effect on NHBE cells. GCG and GA are the main active ingredients in tea for chemoprevention of lung cancer by PCA.

关 键 词：主成分分析 超高效液相色谱 GSH GSSG 

分 类 号：R285.5[医药卫生—中药学]