龙葵对人结肠癌RKO细胞粘附、移动和侵袭的影响  

Effect of Solanum nigrum on Human Colon Carcinoma RKO Cells

作　　者：胡兵[1] 安红梅[1] 沈克平[1] 史秀峰[1] 邓珊[1] 魏蒙蒙[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中药材》2013年第6期958-961,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81273726);上海市中医药事业三年行动计划首批重大研究项目(ZYSNXD-CC-ZDYJ017);国家中医管理局中医肿瘤重点学科合作项目(LHZLK-1107)

摘　　要：目的:研究龙葵对人结肠癌RKO细胞粘附、移动及侵袭的影响。方法:不同浓度龙葵作用RKO细胞,CCK-8(Cell Counting Kit-8,CCK-8)法检测龙葵对RKO细胞增殖作用,CytoSelectTM48-Well Cell Adhesion Assay检测RKO细胞与基质粘附能力,划痕实验检测RKO细胞移动能力,CytoSelectTM24-Well Cell Invasion Assay检测RKO侵袭能力。结果:终浓度400～1600μg/mL龙葵可以显著抑制RKO细胞增殖,终浓度100～400μg/mL龙葵可以抑制RKO细胞粘附、移动及侵袭能力。结论:龙葵可以抑制RKO细胞增殖,降低RKO细胞粘附、移动及侵袭能力。To evaluate the effect of Solanum nigrum on adhesion, migration and invasion in human colon carcinoma RKO cells. Methods ： RKO cells were treated with different dose of Solanum nigrum. Cell proliferation was detected by CCK-8 assay. Cell adhesion was observed with CytoSelectTM 48-Well Cell Adhesion Assay. Cell migration was detected with scratch assay. Cell invasion was analyzed by CytoSelect^TM 24-Well Cell Invasion Assay. Results： At final concentration of 400 - 1 600 μg/mL, Solanum nigrum significantly inhibited proliferation of RKO cells in a dose-dependent manner. At final concentration of 100 -400 μg,/mL, Solanum nigrum significantly inhibited adhesion, migration and invasion in RKO ceils. Conclusion：Solanum nigrum may inhibit the proliferation, adhesion, migration and invasive abilities in RKO ceils. The present study provides new insight into the application of Solanum nigrum for colon carcinoma treatment that are worthy of further study.

关 键 词：结肠癌 龙葵 增殖 粘附 移动 侵袭 

分 类 号：R285.5[医药卫生—中药学]