苹果‘金冠’AGO1基因克隆及与发育相关miRNA表达分析  

Clone and expression of AGO1 gene and development-related miRNA in apple(Golden Delicious)

作　　者：鹿游[1] 马超[1] 孟冬[1] 李天忠[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农业大学学报》2013年第5期69-76,共8页Journal of China Agricultural University

基　　金：科技部科技基础性工作专项(2012FY110100)

摘　　要：为明确miRNA沉默靶基因的调控机理,克隆AGO1蛋白基因并了解其作为沉默复合物核心成员在miRNA沉默通路中的作用,本研究在分析‘金冠’苹果基因组的基础上,以‘金冠’叶片cDNA为模板,克隆获得了3 234bp AGO1基因全长,蛋白分子质量为119ku,等电点为9.41,包含2个保守的AGO蛋白特征结构域:PAZ和Piwi区,命名为MdAGO1。苹果‘金冠’不同组织MdAGO1基因及13种与发育相关miRNA实时定量PCR分析结果表明:1)MdAGO1在花和果实中表达量均高于叶片;2)miRNA在果实和花中表达量均较高,叶片相对较低。MdAGO1与13种发育相关miRNA表达一致,在苹果中MdAGO1可能与miRNA协同作用调控植物的生长发育。As the internal component of RNA induced silencing complex,ARGONAUTE（AGO） protein palys an important role in all known small RNA-directed regulatory pathways. In this study, the target cDNA clone of AGO1 was isolated from library prepared from Golden Delicious using RT-PCR technique. The cDNA was 3 234 bp , and would encode a protein of 1 078 amino acid with a molecular mass of 119 ku and theoretical isoelectric point of 9.41. The protein contained 2 conserved domains, PAZ and Piwi. Quantitative real-time PCR analysis revealed that Md-AG01 was higher in flower and fruit than leaf. The aboundance of detected decelopmet related miRNA were positively correlated to Md- AGO1. Md-AGO1 and miRNA may regulate the plant growth and development together in apple.

关 键 词：苹果 AGO1 基因克隆 发育相关miRNA 表达分析 

分 类 号：S661.1[农业科学—果树学]