HPLC法同时测定精制冠心颗粒中芍药苷与丹酚酸B含量  

Simultaneous Determination of Paeoniflorin and Salvianolic Acid B in Jingzhi Guanxin Granules by HPLC

作　　者：王俊杰[1] 刘弘[1] 于天杰[1] 张玲昂[1] 张海峰[1] 

机构地区：[1]解放军第152中心医院,河南平顶山467000

出　　处：《中国药师》2013年第8期1175-1177,共3页China Pharmacist

摘　　要：目的:建立HPLC法同时测定精制冠心颗粒中芍药苷、丹酚酸B含量,为质量控制提供技术支持。方法:采用Wondasil^(TM)C_(18)色谱柱(150 mm×4.6 mm,5μm);流动相为0.1%磷酸溶液-乙腈体系,线性梯度洗脱,流速0.8 m1.min^(-1),柱温3℃,检测波长芍药苷(230 nm)、丹酚酸B(286 nm)。结果:芍药苷、丹酚酸B进样量分别在0.430～5.375μg、0.416～4.160μg范围内线性关系良好(R^2≥0.999 6),加样回收率分别为100.61%和101.17%,RSD分别为1.12%和1.65%。结论:本法简便可靠,重复性好,可用于该制剂的含量测定。Objective：To establish an HPLC method for the simultaneous determination of paeoniflorin and salvianolic acid B in Jingzhi Guanxin granules.Method：A WondasilTM C_（18） column（150 mm×4.6 mm,5μm） was used.The mobile phase consisted of 0.1%phosphoric acid and acetonitrile with linear gradient elution.The flow rate was 0.8 ml？min^（-1）,the column temperature was 30℃,and the detection wavelength was set at 230nm for paeoniflorin and 286 for salvianolic acid B.Result：Paeoniflorin and salvianolic acid B was in a good linear relationship within the range of 0.430-5.375μg and 0.416-4.160μg（R^2≥0.9996） with the recovery of 100.61%（RSD=1.12%） and 101.17%（RSD=1.65%）,respectively.Conclusion：The developed method is simple,accurate and reliabled with good repeatability,and can be used to control the quality of Jingzhi Guanxin granules.

关 键 词：高效液相色谱法 精制冠心颗粒 芍药苷 丹酚酸B 

分 类 号：R927.2[医药卫生—药学]