输精管复通后大鼠睾丸生精细胞凋亡状况的研究  

STUDY OF APOPTOSIS IN THE MALE RAT SPERMATOGENIC CELLS AFTER VASOSTOMY

作　　者：董强[1] 杨宇如[1] 张卫东[1] 李虹[1] 魏强[1] 官鹏[2] 黄明孔[3] 

机构地区：[1]华西医科大学附属第一医院泌尿外科 [2]华西医科大学法医学院病理研究室 [3]四川省计划生育科学研究所

出　　处：《中国修复重建外科杂志》2000年第5期298-300,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家计划生育委员会科研基金!资助项目 (1998-2 -1)

摘　　要：目的　研究输精管阻断所致生精细胞凋亡状况在输精管复通后的变化。方法　以雄性 SD大鼠为研究对象 ,建立输精管阻断及阻断后复通模型 ,采用 DNA缺口原位标记凋亡细胞方法 ,观察大鼠生精细胞在输精管复通后 4、8、12及 16周的凋亡状况。结果　生精细胞凋亡逐渐减轻 ,至复通后 8周开始显著少于阻断组 (P<0 .0 5 ) ,但 8周和 12周仍显著多于阻断前水平 (P<0 . 0 5 ) ,至 16周凋亡细胞数接近阻断前水平 (P>0 . 0 5 )。结论输精管阻断所致生精细胞凋亡具有可逆性 ,复通后凋亡可恢复至阻断前水平。Objective To investigate the effect of vasostomy on apoptosis in the male rat spermatogenic cells after vasoligation. Methods Model of vasoligation and vasostomy in male rat was established, and then ter- minal deoxynucleotidyl transferase-mediated dUTP nick labelling technique to detect the apoptosis of spermato- genic cells at 4, 8, 12, 16 weeks after vasostomy. Results The number of apoptotic cells in vasostomy group was significantly lower than that of vasoligation group since 8 weeks after vasostomy( P<0 05) . The number of apop- totic cells in 8 and 12 weeks after vasostomy were significantly higher than that in prevasoligation( P<0 05) . 16 weeks after vasostomy, the number of apoptotic cells restored to the level same as that in prevasoligation stage. Conclusion Vasostomy can reverse the apoptosis of spermatogenic cells due to vasoligation.

关 键 词：输精管复通 生精细胞 睾丸 细胞凋亡 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]