壁虎多肽混合物对人食管癌EC109细胞生长的抑制作用  

Inhibitory effect of Gecko peptides mixture on growth of human esophageal squamous carcinoma cell line EC109 cells

作　　者：徐新丽[1] 王建刚[1] 李瑞芳[1] 李世朋[1] 邱相君[1] 段冷昕[1] 

机构地区：[1]河南科技大学医学院药理学与分子生物学重点实验室,河南洛阳471003

出　　处：《中国临床药理学杂志》2013年第8期602-604,共3页The Chinese Journal of Clinical Pharmacology

基　　金：河南省医学科技攻关基金资助项目(200903109)

摘　　要：目的研究壁虎多肽混合物(GPM)对人食管癌EC109细胞的生长抑制作用及其机制。方法用MTT法检测GPM对细胞增殖的影响;Hoechst 33258荧光染色法观察GPM对细胞形态的影响;免疫细胞化学技术检测细胞内Bcl-2、Caspase-3的表达。结果 GPM抑制了EC109细胞的生长,作用呈时间和剂量依赖性。GPM作用24 h,EC109细胞出现明显的凋亡,且Bcl-2表达降低而Caspase-3表达增高。结论 GPM可以抑制人食管癌EC109细胞增殖并诱导其凋亡,其机制可能与GPM降低食管癌EC109细胞Bcl-2表达而增加Caspase-3表达有关。Objective To explore the effect of Gecko peptides mixture（GPM） on the growth of human esophageal squamous carcinoma cell line EC109 cells and its mechanism.Methods The EC109 cells were cultured in vitro and the inhibitory effect of GPM on their proliferation was investigated by MTT assay.The apoptosis were observed by Hoechst 33258 fluorescence staining.The expression of Bcl-2 and Caspase-3 in EC109 cells were detected by using immunohistochemical method.Results The growth of EC109 cells was inhibited in dose and time dependent manners.Treatment with GPM for 24 hours EC109 cells induced typical apoptotic morphological features,decreased Bcl-2 protein levels while increased Caspase-3 expression.Conclusion GCPs exerts anti-tumor effects in vitro by inducing apoptosis,decreasing the Bcl-2 expression and increasing Caspase-3 expression.

关 键 词：壁虎多肽混合物 食管癌 细胞凋亡 BCL-2 CASPASE-3 

分 类 号：R285.6[医药卫生—中药学]