老年脑出血患者相关感染调节性T细胞与辅助性T细胞表达的分析  

Expression of regulatory T cells and helper T cells in senile cerebral hemorrhage patients complicated with infections

作　　者：赵宏[1] 隋欣[1] 高峰[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院神经二科,黑龙江齐齐哈尔161000

出　　处：《中华医院感染学杂志》2013年第16期3838-3839,3842,共3页Chinese Journal of Nosocomiology

基　　金：黑龙江省科学技术基金项目(9232012Y1079)

摘　　要：目的探讨老年脑出血合并相关感染患者的调节性T细胞与辅助性T细胞的表达。方法对医院2009年2月-2010年2月60例老年脑出血合并感染患者(观察组)和同期的60例老年脑出血无感染患者(对照组)的调节性与辅助性T细胞的表达采取间接免疫荧光法进行测定。结果通过两组的对比分析,观察组患者的调节性T细胞中的CD4+、CD25+和CD4+、CD25+Foxp3水平含量(15.44±4.56)%、(3.97±0.56)%明显的高于对照组的CD4+、CD25+和CD4+、CD25+Foxp3水平含量(12.32±3.45)%、(3.23±0.45)%,差异有统计学有意义(P<0.05);观察组辅助性T细胞中的CD3+、CD4+及CD4+/CD8+比值的(54.34±8.67)%、(30.24±5.35)%、1.24±0.36均明显的低于对照组的(66.72±12.34)%、(37.44±6.34)%、1.78±0.46,而CD8+的(28.45±4.33)%含量明显的高于对照组的(20.23±3.46)%,差异有统计学意义(P<0.05)。结论老年脑出血合并相关感染患者外周血中的调节性T细胞与辅助性T细胞得到异常的表达,可以作为临床中诊断该病的一种新指标,具有重要的临床意义。OBJECTIVE To explore the expression of regulatory T cells and helper T cells in the senile cerebral hemorrhage patients complicated with infections.METHODS Totally 60cases of senile cerebral hemorrhage patients complicated with infections,who were enrolled in the hospital from Feb 2009to Feb 2010,were chosen as the observation group,while 60cases of senile cerebral hemorrhage patients without infections were set as the control group,then the expression of regulatory T cells and helper T cells was determined by using indirect immune fluorescence method.RESULTS By the contrast of the two groups,the levels of CD4＋,CD25＋and CD4＋,CD25＋Foxp3in the regulatory T cells of the observation group were respectively（15.44±4.56）% and（3.97 ±0.56）%,significantly higher than（12.32±3.45）% and（3.23±0.45）% of the control group,the difference was significant（P0.05）;the levels of CD3＋,CD4＋,and CD4＋/CD8＋in the helper T cells of the observation group were respectively（54.34±8.67）%,（30.24±5.35）%,and 1.24±0.36,significantly lower than（66.72 ±12.34）%,（37.44±6.34）%,and 1.78±0.46of the control group,however,the level of CD8＋of the observation group was（28.45±4.33）%,significantly higher than（20.23±3.46）% of the control group,the difference was statistically significant（P0.05）.CONCLUSION The abnormal expression of regulatory T cells and helper T cells in the peripheral blood of the elderly patients with cerebral hemorrhage complicated with infections can serve as a new indicator in the diagnosis of such disease,and it is of great clinical significance.

关 键 词：脑出血合并相关感染 调节性T细胞 辅助性T细胞 表达 

分 类 号：R181.32[医药卫生—流行病学]