甜菜耐低温种质筛选和苗期耐冷性鉴定  

Screening of Sugar Beet Low Temperature Tolerance Germplasm and Identification on Seedling Cold Hardiness

作　　者：丁广洲[1,2,3] 陈丽[1,2] 赵春雷[1,2] 盖志佳[3] 于雪[3] 王希[1,2] 贾海伦[1,2] 马凤鸣[3] 陈连江[1,2] 

机构地区：[1]中国农业科学院甜菜研究所,黑龙江哈尔滨150080 [2]黑龙江省普通高校甜菜遗传育种重点实验室/黑龙江大学,哈尔滨150080 [3]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《种子》2013年第8期1-6,共6页Seed

基　　金：国家甜菜产业技术体系建设项目资助(编号:CARS-210101)

摘　　要：利用人工气候箱,采取耐低温发芽筛选方法,对50份二倍体甜菜种质进行低温胁迫处理,筛选出耐低温的种质12份,芽期耐冷指数≥0.76。通过苗期耐寒性鉴定发现,这12份种质的苗期耐冷能力与芽期基本一致,但个体间对低温的反应速度及持久性表现存在差异,其中,6份资源具有更强的苗期耐冷能力。试验证明,甜菜种质苗期冷害的生理临界温度为3～6℃,在该低温阈值下,耐冷性强的种质对温度变化不太敏感,而耐冷性弱的资源对温度变化较为敏感。1～2℃是甜菜冷害的发生温度,耐冷性强的资源表现为停滞生长,子叶皱缩卷曲,真叶和下胚轴出现似烫伤表现,逐渐死亡;耐冷性弱的资源受冷害症状较早出现,很快发生畸变和死亡。Using artificial climate box, to take the resistance to low temperature germination screening methods, 50 diploid sugar beet germplasm resources were tested. Chilling tolerance index in Bud stage of 12 low temperature resistant germplasm resources is more than 0.76. it was found that the performance of 12 diploid materials resistance to cold damage were similar to Bud stage through appraisal by seedling cold tolerance, but speed and persistent of individual response to low temperature were different. Six materials had a greater tolerance to seedling cold treatments. Experiment proved that seedling cold damage threshold temperature of sugar beet was 3 -6 ℃, under this temperature range, strong cold-endurance germplasm was not sensitive, but weak cold-endurance was sensitive. Cold extreme temperature of diploid materials was 1 - 2 ℃, growth of strong cold-endurance germplasm was stagnant;Cotyledon became shrunk and curly;the leaves and hypocotyl seemed scalded, gradual to death. For weak cold-endurance materials, Chilling injury symptoms appeared earlier, and distortion and death appeared soon.

关 键 词：甜菜 种质 耐低温发芽 苗期耐冷性鉴定 

分 类 号：S566.3[农业科学—作物学]