番茄黄化曲叶病毒病抗病基因的PCR检测  

Detection of Resistant Gene to Tomato Yellow Leaf Curl Virus Disease in Tomato by PCR

作　　者：魏光普[1] 孙瑞芬[2] 尚春明[3] 贾晋[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,内蒙古包头041010 [2]内蒙古农牧业科学院,内蒙古呼和浩特010031 [3]包头市农业科学研究所,内蒙古包头014010

出　　处：《种子》2013年第8期25-27,32,共4页Seed

基　　金：内蒙古自然科学基金(编号:2009 BS 0303);内蒙古科技大学产学研合作项目“耐低温、高光效番茄育种材料筛选及新品种选育”(编号:PY-201116);包头市科技普及项目“包头市番茄黄化曲叶病毒病防治知识的普及”(编号:2012 S 2008-6)

摘　　要：番茄黄化曲叶病毒病是一种严重威胁全世界番茄生产的病害,培育抗病品种已成为防治该病害的主要技术手段。番茄黄化曲叶病毒病抗性基因Ty-1和Ty-3为不完全显性遗传,本研究采用PCR技术,对来自于内蒙古包头市农业科学研究所的11份番茄抗病、耐病及感病育种材料进行了抗病基因Ty-1及Ty-3的检测,并对Ty-1抗性基因进行TaqⅠ酶切。结果发现,11份育种材料中,材料3和材料7中含有Ty-1及Ty-3抗性基因,材料1、材料4、材料5及材料7中含有Ty-3基因,其余材料无抗病基因。研究结果对准确鉴定番茄育种材料的黄化曲叶病抗病基因,抗病育种材料的辅助选择及缩短育种周期具有重要意义。Tomato yellow leaf curl virus disease is a serious threat to tomato production worldwide, breeding resistant varieties has become the main technical means to control the disease. The tomato＇ s resistance gene Ty-1 and Ty-3 are incomplete dominant inheritance. This study detected resistance gene Ty-1 and Ty-3 in tomato materials of resistant and susceptible to Tomato Yellow Leaf Curl Virus by PCR, and used Taq I restriction enzyme to digest Ty-1 gene. The results showed there were Ty-1 and Ty-3 resistance gene in material 3 and materials 7, and were Ty-3 resistance gene in material 1, material 4, material 5 and materials 7, no resistance gene detected from remaining materials. The research work will provide guidance to detect accurately resistance gene, assist selection of breeding material and shorten the period and raise the efficiency in breeding.

关 键 词：番茄黄化曲叶病毒病 抗病基因 PCR 

分 类 号：S641.2[农业科学—蔬菜学]