梨不同嫁接组合嫁接愈合过程中生理动态变化研究  

Study on the Physiological Dynamic Change of Different Graft Combinations in Pear During the Grafted Healing Process

作　　者：孙华丽[1] 宋健坤[1] 李鼎立[1] 王然[1] 

机构地区：[1]青岛农业大学园艺学院,山东青岛266109

出　　处：《北方园艺》2013年第16期25-29,共5页Northern Horticulture

基　　金：国家现代梨产业技术体系资助项目(nycytx-29-06)

摘　　要：以亲和性不同的2种嫁接组合‘鸭梨/豆梨’、‘OHF51/豆梨’为试材,研究了嫁接口部位在嫁接愈合过程中其多酚氧化酶(PPO)活性、苯丙氨酸解氨酶(PAL)活性、酚类物质含量、可溶性糖含量、可溶性蛋白含量、木质素含量等指标的变化情况,以期探究梨嫁接亲和的机理。结果表明:2个嫁接组合的PPO、PAL活性变化趋势基本一致,嫁接亲和性较好的‘鸭梨/豆梨’组合的PPO和PAL活性最高峰值均高于亲和性较差的‘OHF51/豆梨’组合;2个组合的多酚含量相近,且变化趋势基本相同,在愈合过程中总体呈下降趋势;‘鸭梨/豆梨’组合的可溶性糖含量在愈合过程前期高于‘OHF51/豆梨’组合,但后期下降较快;2个组合的可溶性蛋白含量的变化趋势基本一致,总体呈现先升高后降低的过程,嫁接愈合后期基本恢复到初始水平;‘鸭梨/豆梨’组合木质素含量在嫁接愈合初期是逐渐升高的,后期下降后上升。而‘OHF51/豆梨’嫁接组合在嫁接前期木质素含量一直呈下降趋势,后期木质素含量逐渐升高。Taking two different graft combinations of ＇Yali/P.calleryana Dcne＇ and ＇OHF51/P.calleryana Dcne＇ as materials,the physiological dynamic change including polyphenol oxidase（PPO） and phenylalnine ammonialyase（PAL） activity,polyphenol,soluble sugar,soluble protein and lignin content during graft healing process were studied,in order to explore the mechanism of graft compatibility.The results showed that the change trend of activity of PPO and PAL were basically the same.The highest peak value of PPO activity and PAL in ＇Yali/P.calleryana Dcne＇ combination with a good affinity were higher than that in ＇OHF51/P.cvalleryana Dcne＇ combination with a poor affinity;the polyphenol content in two combinations showed declining trend on the whole with a similar change curve and there was no sensible difference in content between two combinations;the soluble sugar content in ＇Yali/P.calleryana Dcne＇ combination was higher than that in ＇OHF51/P.calleryana Dcne＇ combination during most of the period,but declined rapidly at the late stage;the change trend of soluble protein content in two combinations was basically the same and showed the process of first increased and then decreased,at the late stage of healing process,recovered to the normal level;the lignin content in ＇Yali/P.calleryana Dcne＇ combination was gradually increased at early stage of graft healing process,while in ＇OHF51/P.calleryana Dcne＇ combination,the lignin content had been declining at first stage,then gradually increased at late stage.

关 键 词：梨 嫁接亲和性 愈合过程 生理动态变化 

分 类 号：S661.2[农业科学—果树学]