外源Ca^(2+)浓度对PEG6000模拟干旱处理下水稻生理活性的影响  

The Influence of Exogenous Ca^(2+) on the Physiology of Oryza sativa L. Under PEG6000 Simulated Drought

作　　者：隋亚珍[1] 刘杨[1] 许萌萌[1] 刘思雪[1] 尉荣蓉[1] 赵昕[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《生物技术通报》2013年第8期88-93,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30900771);北京市自然科学基金项目(5102006)

摘　　要：以野生型日本晴水稻为试验材料,在水培的条件下用PEG6000模拟干旱处理的方法研究外源Ca2+浓度对干旱胁迫下野生型水稻生理活性的影响。试验结果显示,干旱处理后,植物体内发生了一系列的变化来提高对自身的保护作用,MDA、脯氨酸、叶绿素含量均增加,SOD、POD活力升高。在轻度干旱(PEG=10%)的情况下,Ca2+浓度为1 mmol/L时SOD、POD活力升高、叶绿素增加最为明显,脯氨酸和MDA的含量随着Ca2+浓度的升高而增加;在严重干旱(PEG=20%)的情况下,脯氨酸、叶绿素含量显著增加,并且随着Ca2+浓度的升高积累量逐渐升高,在Ca2+浓度为5 mmol/L时达到最高,而SOD、POD活力在Ca2+浓度为1mmol/L时最高。试验结果显示,在培养液中加入适当的钙离子有助于提高水稻对于不同程度干旱胁迫的耐受性。In this study,the wild type of Oryza.sativa L.was used as the experimental subject.Using water culture method,under PEG6000 simulating drought condition,the physiology indexes of rice were measured,to understand the effect of Ca2＋ on drought.The experiment result showed that,under the osmotic stress,a series of changes happened against the stress,including MDA,SOD,POD,proline,chlorophyll,the contents of which were increased.Under mild desiccation（PEG=10%）,when the concentration of Ca2＋ was 1 mmol/L,the increase of SOD,POD and chlorophyll were most visible,meanwhile the contents of proline and MDA increased with the concentration of Ca2＋.Under severe desiccation（PEG=20%）,the contents of proline and chlorophyll were remarkably raised,with the increased of concentration of Ca2＋,and reached the maximum when[Ca2＋] equals to 5 mmol/L.On the other hand,the activities of SOD and POD reached the maximum at[Ca2＋] equals to 1 mmol/L..

关 键 词：水稻 干旱胁迫 钙离子 抗氧化酶 叶绿素 MDA 脯氨酸 

分 类 号：S511[农业科学—作物学]