DNA条形码鉴别内蒙古地区啮齿动物  

Identification of Rodents by DNA Barcoding in Inner Mongolia Area

作　　者：常子丽[1] 刘芳[1] 王建军[1] 李建云[1] 胡艳红[1] 武正华[1] 王浩辉[1] 李科[1] 杨志羡[1] 马志东[1] 范蒙光[1] 张忠兵[1] 

机构地区：[1]内蒙古自治区地方病防治研究中心,呼和浩特010031

出　　处：《生物技术通报》2013年第8期94-98,共5页Biotechnology Bulletin

基　　金：2012年卫生公益性行业科研专项(201202021)

摘　　要：为探究应用DNA条形码技术对啮齿动物进行分子生物学鉴定的可行性,检测了内蒙古地区7种啮齿动物的59条线粒体COI基因部分序列,用MEGA5.0软件计算GC含量、遗传距离,采用邻接法(NJ)构建分析系统发育树。结果发现,所有序列富含AT,大部分鼠种都有特定的氨基酸组分。种内平均遗传距离为0.6%,不同鼠种间平均遗传距离为23.1%,NJ进化树能清楚的区分7个不同的类群。所以认为通过COI基因应用DNA条码技术可以鉴别内蒙古地区的啮齿动物。To study the feasibility of DNA barcoding in the identification of rodents.59 specimens of 7 species were sequenced（barcoded）for a 657 bp region of the mitochondrial cytochrome oxidase subunit I gene（COI）in Inner Mongolia Area,China.The GC content and genetic distance was calculated using MEGA5.0,and the phylogenetic trees were constructed with Neighbor-Joining（NJ）method.The results showed that AT contents was larger in all sequences,mostly species had specified amino acid component.The average intraspecific genetic distance（K2P）was 0.6% and the average interspecific genetic distance was 23.1%.A Neighbor-Joining tree showed 7 major clusters apparent.We conclude that COI sequencing of DNA barcoding can be used to identify rodents species in Inner Mongolia.

关 键 词：DNA条形码 啮齿动物 COI基因 

分 类 号：Q958[生物学—动物学]