香蕉枯萎病拮抗放线菌1-g-59的筛选与鉴定  

Screening and Identification of Antagonistic Actinomyces 1-g-59 Against Fusarium oxysporium f. sp. cubense

作　　者：刘小玉[1,2] 周登博[1,2] 谭昕[2] 高祝芬[2] 陈波[1,2] 黄霄[1,2] 张锡炎[2] 

机构地区：[1]海南大学园艺园林学院,海口571737 [2]中国热带农业科学院生物技术研究所,海口571101

出　　处：《生物技术通报》2013年第8期124-129,共6页Biotechnology Bulletin

基　　金：现代农业产业技术体系建设专项资金(CARS-32)

摘　　要：通过对海南美台、南宝蕉园香蕉根际土壤的分离和筛选,获得5株对FOC 4具有拮抗作用的放线菌,其中以菌株1-g-59活性最强。根据进行形态特征、培养特征、生理生化特征、细胞壁化学成分分析、16S rDNA序列和系统发育分析等研究,鉴定菌株1-g-59为Streptomyces lunalinharesii。盆栽试验结果表明,菌株1-g-59发酵液对香蕉枯萎病具有较强的防治作用,防治效果达64.9%。Five antagonistic strains against Fusarium oxysporumf.sp.cubense were isolated and screened from the rhizosphere soil of the banana garden in Meitai and Nanbao,strain 1-g-59 possessed the most prominent bioactivity.By studying the morphology,cultural,physiological and biochemical properties,cell wall components analysis,16S rDNA and phylogenetic tree,the results showed that strain 1-g-59 was identified as Streptomyces lunalinharesii.Pot experiment showed that control effects of the fermentation filtrate of strain 1-g-59 on banana fusarium wilt disease was 64.9%.

关 键 词：香蕉枯萎病 拮抗放线菌 筛选 鉴定 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]